Centre of Agricultural Biochemistry and Biotechnology, University of Agriculture, Jail Road, Faisalabad, 38040, Pakistan.
National Institute for Genomics and Advanced Biotechnology, National Agriculture Research Centre, Park Road, Islamabad, 45500, Pakistan.
Mol Biol Rep. 2023 Nov;50(11):8799-8808. doi: 10.1007/s11033-023-08425-1. Epub 2023 Sep 2.
Seeds of super basmati were mutagenized with different ethyl methane sulphonate (EMS) doses for creating genetic variability.
A total of 48 randomly selected putative EMS mutants of super basmati were analyzed to dissect the genetic diversity by using 25 SSR primers located on twelve chromosomes of rice. SSRs analysis revealed that wide-range of genetic diversity is present among mutants of super basmati. A sum of 91 alleles were identified, out of these, 82 alleles were polymorphic and the rest of nine alleles were monomorphic in nature. The range of allele number was 2-10 with mean of 3.64 alleles/locus. The value of polymorphic information content was range between 0.039 (RM5) and 0.878 (RM44) with mean of 0.439 for each locus. A number of polymorphic markers showed unique bands of various sizes ranges from 75 to 1000 bp, during genetic dissection of mutant population. Dendrogram divided whole mutant population into four major groups. Phylogenic analyses revealed that 40-96%genetic similarity is present among individuals of mutant population.
It is concluded that EMS induced genetic variability and SSRs markers (RM44, RM154, RM1, RM252, RM334, RM487, RM110 and RM257) could be handy for the selection of rice mutants as parents for functional genomic and molecular breeding program.
超级巴斯马蒂种子经过不同乙基甲烷磺酸乙酯（EMS）剂量的诱变处理，以产生遗传变异。
共分析了 48 个随机选择的超级巴斯马蒂潜在 EMS 突变体，利用分布在水稻 12 条染色体上的 25 个 SSR 引物来剖析遗传多样性。SSR 分析表明，超级巴斯马蒂突变体之间存在广泛的遗传多样性。共鉴定出 91 个等位基因，其中 82 个等位基因为多态性，其余 9 个等位基因为单态性。等位基因数的范围为 2-10，平均值为 3.64 个/位点。多态信息含量值范围在 0.039（RM5）和 0.878（RM44）之间，每个位点的平均值为 0.439。在对突变体群体进行遗传剖析时，一些多态性标记显示出大小范围从 75 到 1000bp 的独特带。聚类图将整个突变体群体分为四个主要组。系统发育分析表明，突变体群体个体之间存在 40-96%的遗传相似性。
总之，EMS 诱导的遗传变异和 SSRs 标记（RM44、RM154、RM1、RM252、RM334、RM487、RM110 和 RM257）可用于选择水稻突变体作为功能基因组和分子育种计划的亲本。
