Okou David T, Steinberg Karyn Meltz, Middle Christina, Cutler David J, Albert Thomas J, Zwick Michael E
Department of Human Genetics, Emory University School of Medicine, 615 Michael Street, Suite 301, Atlanta, Georgia 30322, USA.
Nat Methods. 2007 Nov;4(11):907-9. doi: 10.1038/nmeth1109. Epub 2007 Oct 14.
We developed a general method, microarray-based genomic selection (MGS), capable of selecting and enriching targeted sequences from complex eukaryotic genomes without the repeat blocking steps necessary for bacterial artificial chromosome (BAC)-based genomic selection. We demonstrate that large human genomic regions, on the order of hundreds of kilobases, can be enriched and resequenced with resequencing arrays. MGS, when combined with a next-generation resequencing technology, can enable large-scale resequencing in single-investigator laboratories.
我们开发了一种通用方法——基于微阵列的基因组选择（MGS），它能够从复杂的真核生物基因组中选择并富集目标序列，而无需进行基于细菌人工染色体（BAC）的基因组选择所必需的重复序列阻断步骤。我们证明，数百千碱基规模的大型人类基因组区域可以通过重测序阵列进行富集和重测序。当MGS与新一代重测序技术相结合时，单研究人员实验室也能够进行大规模重测序。
