用于体外检测蛋白酶活性的化学发光探针。
Chemiluminescent probe for the in vitro detection of protease activity.
作者信息
Richard Jean-Alexandre, Jean Ludovic, Romieu Anthony, Massonneau Marc, Noack-Fraissignes Pauline, Renard Pierre-Yves
机构信息
Equipe de Chimie Bio-Organique, Université de Rouen, INSA de Rouen, CNRS UMR 6014, COBRA, IRCOF, 1 rue Lucien Tesnière, Mont-Saint-Aignan Cedex, France
出版信息
Org Lett. 2007 Nov 8;9(23):4853-5. doi: 10.1021/ol702190y. Epub 2007 Oct 16.
A strategy involving the use of a self-immolative linker has been investigated for the chemiluminescent sensing of proteases. The reactive linker enabled the release of a 1,2-dioxetane light precursor. As a proof of principle, caspase-3, a key peptidase involved in apoptosis has been targeted. An in vitro assay has been carried out and proved the decomposition of the linker and the selectivity for caspase-3.
一种涉及使用自毁型连接子的策略已被研究用于蛋白酶的化学发光传感。这种反应性连接子能够释放一种1,2 - 二氧杂环丁烷光前驱体。作为原理验证，参与细胞凋亡的关键肽酶caspase - 3已成为目标。已进行了体外测定，证明了连接子的分解以及对caspase - 3的选择性。
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