头孢菌素化学发光缀合物将β-内酰胺酶检测灵敏度提高了四个数量级。
A cephalosporin-chemiluminescent conjugate increases beta-lactamase detection sensitivity by four orders of magnitude.
机构信息
Department of Bioengineering, University of California, Berkeley, USA.
出版信息
Chem Commun (Camb). 2020 Mar 25;56(24):3516-3519. doi: 10.1039/c9cc09498a. Epub 2020 Feb 26.
Abstract
The expression of beta-lactamases in bacteria is a central cause of drug resistance. In this report, we present a beta-lactamase chemiluminescent probe, termed CCP, which can for the first time detect beta-lactamase activity via chemiluminescence and can detect beta lactamase with a sensitivity that is 4-orders of magnitude higher than the commercially available fluorescent lactamase substrate fluorocillin.
摘要
β-内酰胺酶在细菌中的表达是导致药物耐药性的主要原因。在本报告中，我们提出了一种β-内酰胺酶化学发光探针，称为 CCP，它可以首次通过化学发光检测β-内酰胺酶活性，并且可以检测β-内酰胺酶的灵敏度比市售的荧光内酰胺酶底物氟氯西林高 4 个数量级。
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