2004年苏丹延比奥疫情期间埃博拉出血热的实验室诊断

Laboratory diagnosis of Ebola hemorrhagic fever during an outbreak in Yambio, Sudan, 2004.

作者信息

Onyango Clayton O, Opoka Martin L, Ksiazek Thomas G, Formenty Pierre, Ahmed Abdullahi, Tukei Peter M, Sang Rosemary C, Ofula Victor O, Konongoi Samson L, Coldren Rodney L, Grein Thomas, Legros Dominique, Bell Mike, De Cock Kevin M, Bellini William J, Towner Jonathan S, Nichol Stuart T, Rollin Pierre E

机构信息

World Health Organization Collaborating Center for Arbovirus and Viral Hemorrhagic Fever Reference and Research, Nairobi, Kenya.

出版信息

J Infect Dis. 2007 Nov 15;196 Suppl 2:S193-8. doi: 10.1086/520609.

DOI:10.1086/520609

PMID:17940949

Abstract

Between the months of April and June 2004, an Ebola hemorrhagic fever (EHF) outbreak was reported in Yambio county, southern Sudan. Blood samples were collected from a total of 36 patients with suspected EHF and were tested by enzyme-linked immunosorbent assay (ELISA) for immunoglobulin G and M antibodies, antigen ELISA, and reverse-transcription polymerase chain reaction (PCR) of a segment of the Ebolavirus (EBOV) polymerase gene. A total of 13 patients were confirmed to be infected with EBOV. In addition, 4 fatal cases were classified as probable cases, because no samples were collected. Another 12 patients were confirmed to have acute measles infection during the same period that EBOV was circulating. Genetic analysis of PCR-positive samples indicated that the virus was similar to but distinct from Sudan EBOV Maleo 1979. In response, case management, social mobilization, and follow-up of contacts were set up as means of surveillance. The outbreak was declared to be over on 7 August 2004.

摘要

2004年4月至6月期间，苏丹南部延比奥县报告了一起埃博拉出血热疫情。共采集了36例疑似埃博拉出血热患者的血样，并通过酶联免疫吸附测定（ELISA）检测免疫球蛋白G和M抗体、抗原ELISA以及埃博拉病毒（EBOV）聚合酶基因片段的逆转录聚合酶链反应（PCR）。共有13例患者被确诊感染埃博拉病毒。此外，有4例死亡病例被列为可能病例，因为未采集样本。另有12例患者在埃博拉病毒传播的同一时期被确诊患有急性麻疹感染。对PCR阳性样本的基因分析表明，该病毒与1979年苏丹埃博拉病毒马莱奥毒株相似但又有所不同。作为监测手段，开展了病例管理、社会动员以及接触者追踪。此次疫情于2004年8月7日宣告结束。