蛋白质组学中的激光捕获采样及分析问题
Laser capture sampling and analytical issues in proteomics.
作者信息
Gutstein Howard B, Morris Jeffrey S
机构信息
MD Anderson Cancer Center, 1515 Holcombe Blvd, Box 110, Houston, TX 77030-4009, USA.
出版信息
Expert Rev Proteomics. 2007 Oct;4(5):627-37. doi: 10.1586/14789450.4.5.627.
Abstract
Proteomics holds the promise of evaluating global changes in protein expression and post-translational modification in response to environmental stimuli. However, difficulties in achieving cellular anatomic resolution and extracting specific types of proteins from cells have limited the efficacy of these techniques. Laser capture microdissection has provided a solution to the problem of anatomical resolution in tissues. New extraction methodologies have expanded the range of proteins identified in subsequent analyses. This review will examine the application of laser capture microdissection to proteomic tissue sampling, and subsequent extraction of these samples for differential expression analysis. Statistical and other quantitative issues important for the analysis of the highly complex datasets generated are also reviewed.
摘要
蛋白质组学有望评估蛋白质表达和翻译后修饰对环境刺激的整体变化。然而，在实现细胞解剖分辨率以及从细胞中提取特定类型蛋白质方面存在的困难限制了这些技术的功效。激光捕获显微切割技术为解决组织中的解剖分辨率问题提供了一种方法。新的提取方法扩大了后续分析中鉴定出的蛋白质范围。本综述将探讨激光捕获显微切割技术在蛋白质组学组织采样中的应用，以及随后对这些样品进行提取以进行差异表达分析。还将对分析所生成的高度复杂数据集至关重要的统计和其他定量问题进行综述。
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