Worwood M, Thorpe S J, Heath A, Flowers C H, Cook J D
Department of Haematology, University of Wales College of Medicine, Heath Park, Cardiff.
Clin Lab Haematol. 1991;13(3):297-305. doi: 10.1111/j.1365-2257.1991.tb00285.x.
Two monoclonal antibodies to human ferritin, including one that was coupled to horseradish peroxidase, were lyophilized. These reagents show little loss of activity on reconstitution and demonstrate acceptable stability in the accelerated degradation test. When applied in a simple ELISA for the assay of serum ferritin along with the WHO standard for serum ferritin (80/602) they provide a robust assay with standardized reagents which is potentially suitable for use as a reference assay.
两种抗人铁蛋白单克隆抗体,其中一种偶联了辣根过氧化物酶,被冻干。这些试剂复溶后活性损失很小,并且在加速降解试验中显示出可接受的稳定性。当与世界卫生组织血清铁蛋白标准品(80/602)一起用于简单的酶联免疫吸附测定(ELISA)来检测血清铁蛋白时,它们提供了一种使用标准化试剂的可靠测定方法,有可能用作参考测定方法。