血清铁蛋白的固相酶免疫测定法。
Solid-phase enzyme immunoassay for serum ferritin.
作者信息
Anaokar S, Garry P J, Standefer J C
出版信息
Clin Chem. 1979 Aug;25(8):1426-31.
PMID:378465
Abstract
We describe a solid-phase enzyme immunoassay for ferritin in human serum, with use of a horseradish peroxidase-labeled antibody and a highly sensitive chromogen, 2,2'-azino-di(3-ethyl-benzthiazoline-6-sulfonate). The assay requires only 10 microL of serum per assay, relatively less time and labor than other assys for ferritin, and as little as 10 pg of ferritin can be measured. We believe this assay offers a reliable alternative to radioimmunoassay for serum ferritin.
摘要
我们描述了一种用于检测人血清中铁蛋白的固相酶免疫测定法，该方法使用辣根过氧化物酶标记的抗体和一种高灵敏度的显色剂2,2'-叠氮基-二(3-乙基苯并噻唑啉-6-磺酸盐)。该测定法每次检测仅需10微升血清，与其他铁蛋白检测方法相比，所需时间和人力相对较少，并且能够检测低至10皮克的铁蛋白。我们认为该测定法为血清铁蛋白的放射免疫测定提供了一种可靠的替代方法。
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