Conradie J D, Mbhele B E
S Afr Med J. 1980 Feb 23;57(8):282-7.
We have developed an enzyme-linked immunosorbent assay (ELISA) for determining ferritin in serum. In this assay the solid phase consists of rabbit antiferritin IgG adsorbed onto PVC microtitre plates. Ferritin from standards or samples binds to the solid phase and is then detected by a conjugate consisting of rabbit antiferritin-IgG and horseradish peroxidase. The entire assay can be performed in 5 hours, with good precision. The assay has a useful range of 1 to 64 microgram/l and serum samples therefore have to be diluted 10-fold. The coefficient of variation for 6 samples (1,5-40,1 microgram/l) assayed 12 times on the same plate varied from 4% to 9% (intra-assay). When these 6 samples were assayed on different plates and on different days by two technicians the coefficients of variation varied from 4% to 11% (inter-assay). The lowest detectable level of ferritin was 1 microgram/l. Forty-six serum samples from blood donors were assayed by the method described here (gamma) and compared with results obtained by RIA (alpha). The resulting regression equation was gamma = 1,073 alpha -- 4,33; r = 0,984.
我们已经开发出一种用于测定血清中铁蛋白的酶联免疫吸附测定法（ELISA）。在该测定法中，固相由吸附在聚氯乙烯微量滴定板上的兔抗铁蛋白IgG组成。标准品或样品中的铁蛋白与固相结合，然后通过由兔抗铁蛋白-IgG和辣根过氧化物酶组成的结合物进行检测。整个测定可在5小时内完成，精密度良好。该测定法的有效范围为1至64微克/升，因此血清样品必须稀释10倍。在同一板上对6个样品（1.5至40.1微克/升）进行12次测定，变异系数在4%至9%之间（批内）。当这6个样品由两名技术人员在不同的板上和不同的日期进行测定时，变异系数在4%至11%之间（批间）。铁蛋白的最低可检测水平为1微克/升。用本文所述方法（γ）对46份献血者的血清样品进行测定，并与放射免疫分析法（α）得到的结果进行比较。得到回归方程为γ = 1.073α - 4.33；r = 0.984。
