Vogeser M, Parhofer K G
Institute of Clinical Chemistry, Hospital of the University of Munich, Munich, Gemany.
Exp Clin Endocrinol Diabetes. 2007 Oct;115(9):559-70. doi: 10.1055/s-2007-981458.
In recent years, liquid chromatography tandem mass spectrometry (LC-MS/MS) has emerged as an innovative analytical technology applicable to a wide number of analyses in the endocrinology laboratory. Compared to the "traditional" technique of gas chromatography-mass spectrometry (GC-MS), LC-MS/MS is easier to use and is applicable for a substantially larger number of relevant analytes. With the development of LC-MS/MS, the widespread application of the proven principle of isotope dilution mass spectrometry is now feasible not only in research but also for routine applications. The aim of this review is to explain the basic technical principles of LC-MS/MS, to describe the general characteristics of analytical LC-MS/MS applications and to comprehensively discuss the application of this technology in the field of endocrinology.
近年来,液相色谱串联质谱法(LC-MS/MS)已成为一种创新的分析技术,适用于内分泌实验室的大量分析。与气相色谱-质谱法(GC-MS)这种“传统”技术相比,LC-MS/MS更易于使用,并且适用于大量相关分析物。随着LC-MS/MS的发展,同位素稀释质谱法这一成熟原理的广泛应用现在不仅在研究中可行,而且在常规应用中也可行。本综述的目的是解释LC-MS/MS的基本技术原理,描述分析型LC-MS/MS应用的一般特征,并全面讨论该技术在内分泌学领域的应用。