Sasso Raffaella, Siniscalchi Marcello, d'Ingeo Serenella, Ventriglia Gianluca, Bitetto Candida, Quaranta Angelo
Department of Veterinary Medicine, University of Bari Aldo Moro, 70121 Bari, Italy.
ACV Triggiano S.R.L., Triggiano, 70019 Bari, Italy.
Animals (Basel). 2025 Feb 27;15(5):689. doi: 10.3390/ani15050689.
Thyroid function is commonly assessed in dogs through serum measurements of free thyroxine (FT4) and thyroid-stimulating hormone (TSH). The accurate measurement of thyroid hormones is critical. In particular, the measurement of free thyroxine (FT4) is important for the diagnosis and management of thyroid disorders. Most laboratories measure FT4 using direct analog immunoassay methods. The reliability of the results obtained using these methods has often been questioned. Fifty-nine dogs for which the veterinarian had requested the evaluation of thyroid hormones were included in this study. FT4 and TSH were measured using chemiluminescence enzyme immunoassay (CLEIA). Furthermore, FT4 was also measured by liquid chromatography-tandem mass spectrometry (LC-MS/MS) preceded by ultrafiltration. Comparing FT4 with the two different methods, the results demonstrate that LC-MS/MS, preceded by ultrafiltration, provides more accurate and specific FT4 measurements, particularly in dogs with altered thyroid hormone values. Although CLEIA is cost-effective and widely used for screening, LC-MS/MS is recommended for the more precise assessment of thyroid function.
通常通过血清中游离甲状腺素（FT4）和促甲状腺激素（TSH）的测量来评估犬的甲状腺功能。甲状腺激素的准确测量至关重要。特别是，游离甲状腺素（FT4）的测量对于甲状腺疾病的诊断和管理很重要。大多数实验室使用直接模拟免疫分析方法测量FT4。使用这些方法获得的结果的可靠性经常受到质疑。本研究纳入了59只兽医要求评估甲状腺激素的犬。使用化学发光酶免疫分析（CLEIA）测量FT4和TSH。此外，在超滤之前还通过液相色谱 - 串联质谱法（LC-MS/MS）测量FT4。将两种不同方法测量的FT4结果进行比较，结果表明，在超滤之前使用LC-MS/MS能提供更准确和特异的FT4测量值，特别是在甲状腺激素值改变的犬中。虽然CLEIA具有成本效益且广泛用于筛查，但建议使用LC-MS/MS对甲状腺功能进行更精确的评估。
