苦参碱注射液对胃癌SGC-7901细胞体外侵袭和转移的影响

[Effect of matrine injections on invasion and metastasis of gastric carcinoma SGC-7901 cells in vitro].

作者信息

Dai Zhi-jun, Gao Jie, Wu Wen-ying, Wang Xi-jing, Li Zong-fang, Kang Hua-feng, Liu Xiao-xu, Ma Xiao-bin

机构信息

Department of Oncology, The Second Hospital of Xi'an Jiaotong University, Xi'an 710004, China.

出版信息

Zhong Yao Cai. 2007 Jul;30(7):815-9.

PMID:17944193

Abstract

OBJECTIVE

To investigate the effect of matrine injections on invasion and metastasis of gastric carcinoma SGC-7901 cells in vitro.

METHODS

MTT assay was used to examine the effect of matrine injections on proliferation of SGC-7901 cells after 24, 48, 72, 96 hours treatment; Transwell chamber assay was performed to determine the effect of matrine injection on invasion and migratory capacity of the cells; Effect on adhesion potential of SGC-7901 cells was tested by cell matrigel adhesion assay.

RESULTS

Matrine injec-tions could inhibit the proliferation of SGC-7901 cells, with obvious dose-dependent and time-dependent effects. Matrine injections sig-nificantly inhibited adhesion, invasion and migration capacity of SGC-7901 cells in vitro. The inhibitory rate of them after treatment with 1.5 mg/ml matrine injections for 24 h were (45.32 +/- 3.10)%, (32.66 +/- 2.82)%, (38.35 +/- 3.41)% respectively. After treat-ment of matrine injections (1.5 mg/ml)for 24 h, the expression level of CD44(V6) in SGC-7901 cells was decreased compared with the untreated group.

CONCLUSION

Matrine injections can inhibit the migration, invasion and adhesion capacity of SGC-7901 cells in vitro. The inhibition effect may be related to down-regulating the expression of CD44(V6) protein.

摘要

目的

探讨苦参碱注射液对胃癌SGC - 7901细胞体外侵袭和转移的影响。

方法

采用MTT法检测苦参碱注射液作用24、48、72、96小时后对SGC - 7901细胞增殖的影响；运用Transwell小室法测定苦参碱注射液对细胞侵袭和迁移能力的影响；通过细胞基质胶黏附实验检测其对SGC - 7901细胞黏附能力的影响。

结果

苦参碱注射液可抑制SGC - 7901细胞的增殖，具有明显的剂量依赖性和时间依赖性。苦参碱注射液能显著抑制SGC - 7901细胞在体外的黏附、侵袭和迁移能力。用1.5mg/ml苦参碱注射液处理24小时后，其对黏附、侵袭和迁移能力的抑制率分别为(45.32±3.10)%、(32.66±2.82)%、(38.35±3.41)%。用1.5mg/ml苦参碱注射液处理24小时后，SGC - 7901细胞中CD44(V6)的表达水平与未处理组相比降低。