Yang Xian, Yang Shui-Ping, Zhang Xue
College of Resource and Environment, Southwest University, Chongqing 400716, China.
Yao Xue Xue Bao. 2007 Aug;42(8):877-81.
To establish the method of HPLC-fingerprint analysis for the quality control of Cnidium monnieri L. Cuss., and identify its active constituents by HPLC-MS, 35 batches of samples were analyzed on a Shimadzu C18 column with a gradient of acetonitrile and 0.1% aqueous aceticacid at a flow rate of 1.0 mL x min(-1) and detected at 245 nm and 322 nm. Furthermore, the typical samples were detected by HPLC-DAD-MS under negative ion mode. 35 batches of Cnidium monnieri L. Cuss. samples were classified into four types based on the results of similarity analysis. According to the comparison of the t(R), MS data and UV maximum absorbance (lambda(max)) values with the standards, 8, 7, 4 and 2 coumarins components were identified in four types of Cnidium monnieri L. Cuss. extracts, separately. The method is repeatable and reliable, and it is capable of effectively controlling the quality of Cnidium monnieri L.
建立用于蛇床子质量控制的高效液相色谱指纹图谱分析方法，并通过高效液相色谱-质谱联用技术鉴定其活性成分。采用岛津C18色谱柱，以乙腈和0.1%醋酸水溶液为流动相进行梯度洗脱，流速为1.0 mL·min⁻¹，检测波长为245 nm和322 nm，对35批样品进行分析。此外，在负离子模式下采用高效液相色谱-二极管阵列-质谱联用技术对典型样品进行检测。根据相似度分析结果，将35批蛇床子样品分为四类。通过与对照品比较保留时间（t(R)）、质谱数据和紫外最大吸收波长（λ(max)）值，在四类蛇床子提取物中分别鉴定出8种、7种、4种和2种香豆素类成分。该方法具有重复性和可靠性，能够有效控制蛇床子的质量。
