Chen Yi, Yan Yan, Xie Ming-Yong, Nie Shao-Ping, Liu Wei, Gong Xiao-Feng, Wang Yuan-Xing
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, People's Republic of China.
J Pharm Biomed Anal. 2008 Jul 15;47(3):469-77. doi: 10.1016/j.jpba.2008.01.039. Epub 2008 Feb 6.
A new high-performance liquid chromatographic (HPLC) fingerprinting method was developed for the quality control of Ganoderma lucidum. Twenty-nine batches obtained from three different origins in China were used to establish the fingerprint. The constituents of these samples were separated with a Kromasil C(18) column (250 mm x 4.6 mm, 5 microm) by linear gradient elution using water-acetic acid (100:0.1, v/v) and acetonitrile as mobile phase components at a flow rate of 0.8 ml/min and detector wavelength at 254 nm. Mean chromatograms and correlation coefficients of samples were calculated by the software "Similarity Evaluation System for Chromatographic Fingerprint of TCM". There were 19 common peaks in this fingerprint. Eleven of these common peaks were tentatively identified with reference to literature data based on their liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS) and UV data. This profile was then successfully used to identify and assess the differences among samples from various origins with the aid of similarity analysis. The diverse similarities among different samples indicated that the quality of G. lucidum was not stable and the products from different areas were inconsistent. All results showed that the developed fingerprint assay was specific and could further serve for quality identification and comprehensive evaluation of G. lucidum.
建立了一种用于灵芝质量控制的新型高效液相色谱（HPLC）指纹图谱方法。使用从中国三个不同产地获取的29批样品来建立指纹图谱。这些样品的成分通过Kromasil C(18)柱（250 mm×4.6 mm，5μm）进行分离，采用水 - 乙酸（100:0.1，v/v）和乙腈作为流动相组分进行线性梯度洗脱，流速为0.8 ml/min，检测波长为254 nm。通过“中药色谱指纹图谱相似度评价系统”软件计算样品的平均色谱图和相关系数。该指纹图谱中有19个共有峰。其中11个共有峰根据其液相色谱 - 电喷雾电离 - 串联质谱（LC - ESI - MS）和紫外数据，参考文献资料进行了初步鉴定。然后借助相似度分析，该图谱成功用于识别和评估不同产地样品之间的差异。不同样品之间多样的相似度表明灵芝的质量不稳定，不同地区的产品不一致。所有结果表明，所建立的指纹图谱测定方法具有特异性，可进一步用于灵芝的质量鉴定和综合评价。
