Moioli Eduardo K, Mao Jeremy J
Conf Proc IEEE Eng Med Biol Soc. 2006;2006:2647-50. doi: 10.1109/IEMBS.2006.260861.
Human mesenchymal stem cells (hMSC) have been shown to differentiate into chondrocytes and form cartilage-like tissues when cultured with TGFbeta3 at 10 ng/ml. Previous attempts to engineer cartilage using hMSC have depended on in vitro pre-differentiation in order to form chondrogenic engineered constructs. Such techniques greatly increase the time of implant fabrication and suffer from loss of phenotype upon withdrawal from chondrogenic medium. The present study investigates a tissue engineered construct that includes sustained delivery of TGFbeta3 and induces differentiation of hMSC into chondrocytes in situ using injectable thermosensitive gels.
人骨髓间充质干细胞（hMSC）已被证明，当在10 ng/ml的转化生长因子β3（TGFbeta3）中培养时，可分化为软骨细胞并形成类软骨组织。以往利用hMSC构建软骨组织的尝试依赖于体外预分化，以形成软骨生成工程构建体。这类技术极大地增加了植入物制造的时间，并且在从软骨生成培养基中撤出后会出现表型丧失的问题。本研究调查了一种组织工程构建体，该构建体包括TGFbeta3的持续递送，并使用可注射热敏凝胶原位诱导hMSC分化为软骨细胞。
