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输卵管类肝癌衍生细胞系HEPFT的建立与特性分析,特别涉及甲胎蛋白、凝集素亲和力及组织发生

Establishment and characterization of HEPFT, a cell line derived from hepatoid carcinoma of the fallopian tube, with special reference to alpha-fetoprotein, lectin affinity and histogenesis.

作者信息

Ishiwata Iwao, Yasuda Makoto, Hirano Takashi, Ishikawa Hiroshi

机构信息

Department of Obstetrics and Gynecology, Jikei University School of Medicine, Tokyo, Japan.

出版信息

Hum Cell. 2007 Nov;20(4):119-30. doi: 10.1111/j.1749-0774.2007.00039.x.

Abstract

A cell line designated "HEPFT" was established from a human fallopian tubal hepatoid carcinoma. This line grew well without interruption for 13 months and was subcultivated over 35 times. The cells were spherical and polygonal in shape and showed neoplastic and pleomorphic features such as a bizarre aggregation of chromatin granules, an irregular thickening membrane and multiple large nucleoli. The cells formed epithelial colonies with a jigsaw puzzle-like arrangement and multilayering without contact inhibition. The cells contained moderate to abundant amounts of eosinophilic cytoplasm and were immunohistochemically positive for alpha-fetoprotein. The cells proliferated rapidly, and the population doubling time was about 45 h. The chromosome number showed a wide distribution of aneuploidy. The modal chromosome number was stable in the hyper triploid range and many marker chromosomes were observed. The culture cells produced bile and a large amount of lentil lectin-reactive alpha-fetoprotein. The recently developed bacterial artificial chromosome array comparative genomic hybridization facilitated detailed analysis with high resolution and sensitivity. Different profiles of genomic copy-number abnormalities were demonstrated in various chromosomal regions in HEPFT cells.

摘要

一种名为“HEPFT”的细胞系源自人输卵管肝样癌。该细胞系连续生长良好达13个月,传代超过35次。细胞呈球形和多边形,具有肿瘤性和多形性特征,如染色质颗粒的奇异聚集、不规则增厚的细胞膜和多个大核仁。细胞形成上皮细胞集落,呈拼图样排列且多层生长,无接触抑制。细胞含有中等至丰富量的嗜酸性细胞质,免疫组化检测甲胎蛋白呈阳性。细胞增殖迅速,群体倍增时间约为45小时。染色体数目显示非整倍体分布广泛。众数染色体数目在超三倍体范围内稳定,观察到许多标记染色体。培养细胞产生胆汁和大量扁豆凝集素反应性甲胎蛋白。最近开发的细菌人工染色体阵列比较基因组杂交技术有助于进行高分辨率和高灵敏度的详细分析。在HEPFT细胞的各个染色体区域显示出不同的基因组拷贝数异常图谱。

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