Eleftheriadis Theodoros, Kartsios Charalambos, Yiannaki Efi, Kazila Polizo, Antoniadi Georgia, Liakopoulos Vassilios, Markala Dimitra
Nephrology Department, General Hospital of Serres, Serres, Greece.
Am J Nephrol. 2008;28(1):152-7. doi: 10.1159/000110020. Epub 2007 Oct 19.
Clinical and experimental data indicate a deficient immune response in hemodialysis (HD) patients. zeta-Chain phosphorylation is an early and central event in the process that follows antigen recognition by the T cell antigen receptor (TCR). T cell zeta-chain is downregulated in many chronic inflammatory states, such as cancer, autoimmune disease and chronic infection. HD is also characterized as a chronic inflammatory state. The aim of the present study was to evaluate T cell zeta-chain expression in HD patients.
Thirty-three stable HD patients and 30 healthy volunteers were enrolled into the study. T cell count, the percentage of zeta-chain-positive T cells, as well as T cell zeta-chain mean fluorescence intensity (MFI) were evaluated with flow cytometry. The inflammatory markers C-reactive protein, interleukin-6 and tumor necrosis factor-alpha were measured in the serum by means of ELISA.
All the evaluated markers of inflammation were increased in HD patients. In these patients, T cell zeta-chain MFI was decreased. CD3-epsilon MFI did not differ between the two groups indicating that among the TCR complex constituents, zeta-chain is selectively downregulated.
HD is a state of chronic inflammation. Like in other pathological chronic inflammatory conditions, T cell zeta-chain is downregulated in HD patients. Since zeta-chain plays a key role in the transduction of the signal that follows antigen recognition by the TCR, its downregulation could be responsible for the deficient cellular immune response observed in HD patients.
临床和实验数据表明血液透析（HD）患者存在免疫反应缺陷。ζ链磷酸化是T细胞抗原受体（TCR）识别抗原后过程中的早期核心事件。T细胞ζ链在许多慢性炎症状态下表达下调，如癌症、自身免疫性疾病和慢性感染。HD也具有慢性炎症状态的特征。本研究的目的是评估HD患者T细胞ζ链的表达。
33例稳定的HD患者和30名健康志愿者纳入本研究。采用流式细胞术评估T细胞计数、ζ链阳性T细胞百分比以及T细胞ζ链平均荧光强度（MFI）。通过ELISA法检测血清中的炎症标志物C反应蛋白、白细胞介素-6和肿瘤坏死因子-α。
HD患者所有评估的炎症标志物均升高。在这些患者中，T细胞ζ链MFI降低。两组之间CD3-ε MFI无差异，表明在TCR复合物成分中，ζ链被选择性下调。
HD是一种慢性炎症状态。与其他病理性慢性炎症情况一样，HD患者T细胞ζ链下调。由于ζ链在TCR识别抗原后信号转导中起关键作用，其下调可能是HD患者细胞免疫反应缺陷的原因。
