一种超快速冷冻蛋白质晶体的通用方法。
A general method for hyperquenching protein crystals.
作者信息
Warkentin Matthew, Thorne Robert E
机构信息
Physics Department, Cornell University, Ithaca, NY 14853, USA.
出版信息
J Struct Funct Genomics. 2007 Dec;8(4):141-4. doi: 10.1007/s10969-007-9029-0. Epub 2007 Oct 19.
Abstract
During flash cooling of protein crystals in liquid cryogens, cooling rates are determined by sample size, choice of cooling liquid, and by the thickness of the cold gas layer that forms above the liquid. We describe an experimental protocol for ultra-rapid cooling of protein crystals. This protocol requires no complex apparatus, and yields ice-ring-free diffraction without the use of penetrating cryoprotectants.
摘要
在蛋白质晶体于液态低温介质中进行快速冷却的过程中，冷却速率取决于样品大小、冷却液的选择以及在液体上方形成的冷气层厚度。我们描述了一种用于蛋白质晶体超快速冷却的实验方案。该方案无需复杂设备，且在不使用穿透性冷冻保护剂的情况下能获得无冰环衍射结果。
相似文献
1
A general method for hyperquenching protein crystals.一种超快速冷冻蛋白质晶体的通用方法。
J Struct Funct Genomics. 2007 Dec;8(4):141-4. doi: 10.1007/s10969-007-9029-0. Epub 2007 Oct 19.
2
Heat transfer from protein crystals: implications for flash-cooling and X-ray beam heating.蛋白质晶体的热传递：对快速冷却和X射线束加热的影响。
Acta Crystallogr D Biol Crystallogr. 2003 Apr;59(Pt 4):697-708. doi: 10.1107/s0907444903002713. Epub 2003 Mar 25.
3
High-pressure cooling of protein crystals without cryoprotectants.无冷冻保护剂时蛋白质晶体的高压冷却
Acta Crystallogr D Biol Crystallogr. 2005 Jul;61(Pt 7):881-90. doi: 10.1107/S090744490500836X. Epub 2005 Jun 24.
4
A procedure and double-chambered device for macromolecular crystal flash-cooling in different cryogenic liquids.一种用于在不同低温液体中对大分子量晶体进行闪光冷却的方法和双室设备。
PLoS One. 2020 Sep 25;15(9):e0239706. doi: 10.1371/journal.pone.0239706. eCollection 2020.
5
Macromolecular cryocrystallography--methods for cooling and mounting protein crystals at cryogenic temperatures.大分子低温晶体学——在低温下冷却和安装蛋白质晶体的方法。
Methods. 2004 Nov;34(3):415-23. doi: 10.1016/j.ymeth.2004.03.032.
6
Protein crystallization and structure determination at room temperature in the CrystalChip.在CrystalChip中于室温下进行蛋白质结晶及结构测定。
FEBS Open Bio. 2025 Apr;15(4):532-541. doi: 10.1002/2211-5463.13932. Epub 2024 Nov 21.
7
Hyperquenching for protein cryocrystallography.用于蛋白质低温晶体学的超猝灭
J Appl Crystallogr. 2006 Dec 1;39(6):805-811. doi: 10.1107/S0021889806037484.
8
Solution of protein crystallographic structures by high-pressure cryocooling and noble-gas phasing.通过高压冷冻冷却和稀有气体定相法解析蛋白质晶体结构
Acta Crystallogr D Biol Crystallogr. 2006 Jul;62(Pt 7):687-94. doi: 10.1107/S0907444906014727. Epub 2006 Jun 20.
9
Cryosalts: suppression of ice formation in macromolecular crystallography.冷冻盐：抑制大分子晶体学中的冰晶形成
Acta Crystallogr D Biol Crystallogr. 2000 Aug;56(Pt 8):996-1001. doi: 10.1107/s0907444900007587.
10
Macromolecular crystal annealing: overcoming increased mosaicity associated with cryocrystallography.大分子晶体退火：克服与低温晶体学相关的镶嵌性增加问题。
Acta Crystallogr D Biol Crystallogr. 1998 Jul 1;54(Pt 4):622-8. doi: 10.1107/s0907444997019008.
引用本文的文献
1
Structure of reverse gyrase with a minimal latch that supports ATP-dependent positive supercoiling without specific interactions with the topoisomerase domain.具有最小闩锁的反向拓扑异构酶结构，支持 ATP 依赖性正超螺旋，而无需与拓扑异构酶结构域的特定相互作用。
Acta Crystallogr D Struct Biol. 2023 Jun 1;79(Pt 6):498-507. doi: 10.1107/S2059798323002565. Epub 2023 May 19.
2
Effectiveness of different types of mask in aerosol dispersion in SARS-CoV-2 infection.不同类型口罩在 SARS-CoV-2 感染气溶胶传播中的效果。
Int J Infect Dis. 2021 Aug;109:310-314. doi: 10.1016/j.ijid.2021.06.029. Epub 2021 Jun 17.
3
Computer-controlled liquid-nitrogen drizzling device for removing frost from cryopreserved crystals.计算机控制的液氮喷淋装置，用于去除冷冻保存晶体上的霜。
Acta Crystallogr F Struct Biol Commun. 2020 Dec 1;76(Pt 12):616-622. doi: 10.1107/S2053230X2001420X. Epub 2020 Nov 25.
4
D3R Grand Challenge 2: blind prediction of protein-ligand poses, affinity rankings, and relative binding free energies.D3R 大挑战 2：蛋白质-配体构象、亲和力排序和相对结合自由能的盲预测。
J Comput Aided Mol Des. 2018 Jan;32(1):1-20. doi: 10.1007/s10822-017-0088-4. Epub 2017 Dec 4.
5
Mapping the conformational space accessible to catechol-O-methyltransferase.绘制儿茶酚-O-甲基转移酶可及的构象空间
Acta Crystallogr D Biol Crystallogr. 2014 Aug;70(Pt 8):2163-74. doi: 10.1107/S1399004714012917. Epub 2014 Jul 25.
6
Approaches to automated protein crystal harvesting.蛋白质晶体自动收获方法。
Acta Crystallogr F Struct Biol Commun. 2014 Feb;70(Pt 2):133-55. doi: 10.1107/S2053230X14000387. Epub 2014 Jan 28.
7
Recognition of two distinct elements in the RNA substrate by the RNA-binding domain of the T. thermophilus DEAD box helicase Hera.热球菌 DEAD 框解旋酶 Hera 的 RNA 结合域对 RNA 底物中两个不同元件的识别。
Nucleic Acids Res. 2013 Jul;41(12):6259-72. doi: 10.1093/nar/gkt323. Epub 2013 Apr 25.
8
Crystal structures of Thermotoga maritima reverse gyrase: inferences for the mechanism of positive DNA supercoiling.海洋栖热菌反转酶的晶体结构：正向 DNA 超螺旋化机制的推论。
Nucleic Acids Res. 2013 Jan;41(2):1058-70. doi: 10.1093/nar/gks1073. Epub 2012 Dec 2.
9
First experiences with semi-autonomous robotic harvesting of protein crystals.蛋白质晶体半自动机器人收获的首次经验。
J Struct Funct Genomics. 2011 Jul;12(2):77-82. doi: 10.1007/s10969-011-9103-5. Epub 2011 Mar 23.
10
The Thermus thermophilus DEAD box helicase Hera contains a modified RNA recognition motif domain loosely connected to the helicase core.嗜热栖热菌的DEAD盒解旋酶赫拉包含一个与解旋酶核心松散连接的修饰RNA识别基序结构域。
RNA. 2009 Nov;15(11):1993-2001. doi: 10.1261/rna.1820009. Epub 2009 Aug 26.
本文引用的文献
1
Hyperquenching for protein cryocrystallography.用于蛋白质低温晶体学的超猝灭
J Appl Crystallogr. 2006 Dec 1;39(6):805-811. doi: 10.1107/S0021889806037484.
2
The molecular organization of cypovirus polyhedra.质型多角体病毒多角体的分子结构
Nature. 2007 Mar 1;446(7131):97-101. doi: 10.1038/nature05628.
3
Cryo-cooling in macromolecular crystallography: advantages, disadvantages and optimization.大分子晶体学中的低温冷却：优点、缺点与优化
Q Rev Biophys. 2004 May;37(2):105-19. doi: 10.1017/s0033583504004007.
4
Macromolecular cryocrystallography--methods for cooling and mounting protein crystals at cryogenic temperatures.大分子低温晶体学——在低温下冷却和安装蛋白质晶体的方法。
Methods. 2004 Nov;34(3):415-23. doi: 10.1016/j.ymeth.2004.03.032.
5
Biomolecular cryocrystallography: structural changes during flash-cooling.生物分子低温晶体学：快速冷却过程中的结构变化
Proc Natl Acad Sci U S A. 2004 Apr 6;101(14):4793-8. doi: 10.1073/pnas.0308315101. Epub 2004 Mar 29.
6
Heat transfer from protein crystals: implications for flash-cooling and X-ray beam heating.蛋白质晶体的热传递：对快速冷却和X射线束加热的影响。
Acta Crystallogr D Biol Crystallogr. 2003 Apr;59(Pt 4):697-708. doi: 10.1107/s0907444903002713. Epub 2003 Mar 25.
7
Reversible lattice repacking illustrates the temperature dependence of macromolecular interactions.可逆晶格重排说明了大分子相互作用对温度的依赖性。
J Mol Biol. 2001 Aug 24;311(4):851-62. doi: 10.1006/jmbi.2001.4891.
8
Cool data: quantity AND quality.酷数据：数量与质量。
Acta Crystallogr D Biol Crystallogr. 1999 Oct;55(Pt 10):1641-53. doi: 10.1107/s0907444999008653.
9
Trends and challenges in experimental macromolecular crystallography.实验大分子晶体学的趋势与挑战
Q Rev Biophys. 1996 Aug;29(3):227-78. doi: 10.1017/s0033583500005837.
10
Cryocrystallography.低温晶体学
Structure. 1994 Dec 15;2(12):1135-40. doi: 10.1016/s0969-2126(94)00116-2.
Zotero 插件