Wang Quankai, Li Ying, Dong Lin, Yang Min, Hu Jie, Sun Jinxiu, Xie Guangyun, Xu Jianning
National Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Key Laboratory of Chemical Safety and Health, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
Wei Sheng Yan Jiu. 2011 Sep;40(5):559-63.
To observed the chromosome damage of human bronchial epithelial cells induced by glycidyl methacrylate (GMA).
Chromosome aberration analysis of human bronchial epithelial cells treated with GMA at different dosages (4, 8, 12, 16 and 20 microg/ml), times (1, 2 and 3 times), and phases (10th, 30th genetation) was detected.
In the dosages range from 4 to 20 microg/ml, the aberration rates (3%, 6%, 7%, 11% and 14%) were demonstrated increasingly with the increase of exposure doses, and dose-effect relationship was found. Significant differences were observed when treated with GMA three times (6%, 7% and 10%). Structure aberrations were found in the transformed 10th-generation cells, while number aberrations were mainly manifested in the transformed 30th-generation cells.
The chromosome aberration can be induced by GMA in the human bronchial epithelial cells, from the structure aberration at the beginning to the lack of normal nuclear style.
观察甲基丙烯酸缩水甘油酯（GMA）诱导人支气管上皮细胞的染色体损伤情况。
检测不同剂量（4、8、12、16和20微克/毫升）、不同次数（1、2和3次）及不同传代阶段（第10代、第30代）的GMA处理后人支气管上皮细胞的染色体畸变情况。
在4至20微克/毫升剂量范围内，随着暴露剂量增加，畸变率（3%、6%、7%、11%和14%）呈递增趋势，存在剂量-效应关系。三次处理GMA时观察到显著差异（6%、7%和10%）。在转化的第10代细胞中发现结构畸变，而数量畸变主要表现在转化的第30代细胞中。
GMA可诱导人支气管上皮细胞发生染色体畸变，从开始的结构畸变到正常核型缺失。
