Mannion C, Lynch P B, Egan J, Leonard F C
School of Agriculture, Food Safety and Veterinary Medicine, UCD, Dublin 4, Ireland.
J Appl Microbiol. 2007 Nov;103(5):1386-92. doi: 10.1111/j.1365-2672.2007.03384.x.
Studies were carried out to investigate the survival of Salmonella Typhimurium and Salmonella Derby in pig slurry during summer and winter seasons.
Pig slurry samples collected from a commercial fattening house were inoculated with a broth culture of Salmonella Typhimurium and Salmonella Derby, each at a level of log(10) 5.0 CFU ml(-1) and log(10) 2.0 CFU ml(-1). At the higher inoculum level, S. Typhimurium and S. Derby survived for 34 and 23 days, respectively in the summer, and 58 and 46 days, respectively in the winter. Survival at the lower inoculum level for S. Typhimurium and S. Derby was 19 and 16 days, respectively, in the summer and 24 days for both in the winter.
The survival of S. Typhimurium and S. Derby observed in this study indicates that a 2-month holding period of pig slurry, prior to land spreading, may be adequate if separate storage facilities are provided.
Despite difficulties correlating laboratory studies with on-farm conditions, pig slurry may not represent a major source of transmission of Salmonella spp. in the farm environment in Ireland.
开展研究以调查鼠伤寒沙门氏菌和德比沙门氏菌在夏季和冬季猪粪浆中的存活情况。
从一家商业育肥场采集猪粪浆样本，分别接种鼠伤寒沙门氏菌和德比沙门氏菌的肉汤培养物，接种水平分别为每毫升10的5.0次方CFU和每毫升10的2.0次方CFU。在较高接种水平下，鼠伤寒沙门氏菌和德比沙门氏菌在夏季分别存活34天和23天，在冬季分别存活58天和46天。在较低接种水平下，鼠伤寒沙门氏菌和德比沙门氏菌在夏季分别存活19天和16天，在冬季均存活24天。
本研究中观察到的鼠伤寒沙门氏菌和德比沙门氏菌的存活情况表明，如果提供单独的储存设施，猪粪浆在土地施用前储存2个月可能就足够了。
尽管将实验室研究与农场实际情况相关联存在困难，但在爱尔兰农场环境中，猪粪浆可能并非沙门氏菌传播的主要来源。
