Gutiérrez T, Mulloy B, Black K, Green D H
Microbial and Molecular Biology Department, Scottish Association for Marine Science, Dunstaffnage Marine Laboratory, Oban, Scotland, UK.
J Appl Microbiol. 2007 Nov;103(5):1716-27. doi: 10.1111/j.1365-2672.2007.03407.x.
To partially purify and characterize bioemulsifiers produced by two new marine Halomonas species, TG39 and TG67, and to compare their emulsifying activities with those of commercial emulsifiers.
The production of emulsifiers HE39 and HE67 was achieved from glucose-supplemented marine broth, and recovered by cell removal, concentration by ultrafiltration, precipitation with salt and ethanol, and lyophilization. Purification and chemical analysis revealed both emulsifiers to be glycoproteins of high molecular weight with a notably high content of protein and uronic acids. Physical characterization showed both glycoproteins to effectively emulsify a wide range of food oils under both neutral and acidic pH conditions and withstand acid and high temperature treatment.
The emulsifying activities of these two new glycoprotein emulsifiers were comparable and, under certain conditions, superior to those produced by commercial emulsifiers tested (xanthan gum, gum arabic and lecithin). They show the highest reported emulsifying activities derived from a Halomonas species.
These strains, and the emulsifiers produced, appear to be promising candidates for further development in applications requiring emulsifiers that are natural and compatible to the existing commercial emulsifiers.
对两种新的海洋嗜盐单胞菌TG39和TG67产生的生物乳化剂进行部分纯化和特性鉴定，并将其乳化活性与商业乳化剂的乳化活性进行比较。
从添加葡萄糖的海洋肉汤中生产乳化剂HE39和HE67，通过去除细胞、超滤浓缩、盐析和乙醇沉淀以及冻干进行回收。纯化和化学分析表明，这两种乳化剂均为高分子量糖蛋白，蛋白质和糖醛酸含量显著较高。物理特性表明，这两种糖蛋白在中性和酸性pH条件下均能有效乳化多种食用油，并能耐受酸和高温处理。
这两种新型糖蛋白乳化剂的乳化活性相当，在某些条件下优于所测试的商业乳化剂（黄原胶、阿拉伯胶和卵磷脂）。它们表现出嗜盐单胞菌属报道的最高乳化活性。
这些菌株以及所产生的乳化剂似乎是有前途的候选物，可在需要天然且与现有商业乳化剂兼容的乳化剂的应用中进一步开发。
