Trisrivirat Duangthip, Hughes John M X, Hoeven Robin, Faulkner Matthew, Toogood Helen, Chaiyen Pimchai, Scrutton Nigel S
Department of Chemistry, School of Natural Sciences, EPSRC/BBSRC Future Biomanufacturing Research Hub, BBSRC/EPSRC Synthetic Biology Research Centre SYNBIOCHEM Manchester Institute of Biotechnology, The University of Manchester, Manchester M1 7DN, UK.
School of Biomolecular Science and Engineering, Vidyasirimedhi Inistitute of Science and Technology (VISTEC), Rayong 21210, Thailand.
Synth Biol (Oxf). 2020 Oct 27;5(1):ysaa022. doi: 10.1093/synbio/ysaa022. eCollection 2020.
Successful industrial biotechnological solutions to biofuels and other chemicals production rely on effective competition with existing lower-cost natural sources and synthetic chemistry approaches enabled by adopting low-cost bioreactors and processes. This is achievable by mobilizing as a next generation industrial chassis, which can be cultivated under non-sterile conditions. To increase the cost effectiveness of an existing sustainable low carbon bio-propane production strategy, we designed and screened a constitutive promoter library based on the known strong porin promoter from . Comparative studies were performed between and using the reporter gene red fluorescent protein (RFP). Later studies with a fatty acid photodecarboxylase-RFP fusion protein demonstrated tuneable propane production in and , with an ∼8-fold improvement in yield over comparable isopropyl-β-D-thiogalactoside-inducible systems. This novel set of promoters is a useful addition to the synthetic biology toolbox for future engineering of to make chemicals and fuels.
成功的工业生物技术解决方案用于生物燃料和其他化学品生产，依赖于与现有的低成本天然来源以及通过采用低成本生物反应器和工艺实现的合成化学方法进行有效竞争。这可以通过利用作为下一代工业底盘来实现，其可以在非无菌条件下培养。为了提高现有可持续低碳生物丙烷生产策略的成本效益，我们基于已知的来自[具体来源]的强孔蛋白启动子设计并筛选了一个组成型启动子文库。使用报告基因红色荧光蛋白（RFP）在[具体对象1]和[具体对象2]之间进行了比较研究。后来对脂肪酸光脱羧酶-RFP融合蛋白的研究表明，在[具体对象1]和[具体对象2]中丙烷产量可调，与可比的异丙基-β-D-硫代半乳糖苷诱导系统相比，产量提高了约8倍。这组新的启动子是合成生物学工具箱的有用补充，用于未来对[具体对象]进行工程改造以生产化学品和燃料。
