Morris Andrew J, Smyth Susan S
Division of Cardiovascular Medicine, The Gill Heart Institute, University of Kentucky College of Medicine, Lexington, Kentucky, USA.
Methods Enzymol. 2007;434:89-104. doi: 10.1016/S0076-6879(07)34005-6.
Lysophosphatidic acid (LPA) is a bioactive lipid mediator present in the blood and other biological fluids at physiologically relevant concentrations. In the cardiovascular system, studies using in vitro and in vivo experimental models indicate that LPA stimulates platelet activation, differentiation and migration of vascular smooth muscle cells, and changes in vascular tone. A growing body of evidence suggests that aberrant production and actions of LPA could play an important role in atherothrombotic disease. Hydrolysis of lysophospholipids by the secreted plasma protein autotaxin/lysophospholipase D (lysoPLD) is a major mechanism for generation of LPA in the blood. This chapter describes methods for determining the activity of recombinant autotaxin/lysoPLD using radiolabeled and fluorogenic substrates.
溶血磷脂酸（LPA）是一种生物活性脂质介质，以生理相关浓度存在于血液和其他生物体液中。在心血管系统中，使用体外和体内实验模型的研究表明，LPA可刺激血小板活化、血管平滑肌细胞的分化和迁移以及血管张力的变化。越来越多的证据表明，LPA的异常产生和作用可能在动脉粥样硬化血栓形成疾病中起重要作用。分泌型血浆蛋白自分泌运动因子/溶血磷脂酶D（lysoPLD）对溶血磷脂的水解是血液中LPA生成的主要机制。本章介绍了使用放射性标记和荧光底物测定重组自分泌运动因子/lysoPLD活性的方法。
