羧酸酯酶及其与有机磷农药长期影响的关联。

Carboxylic esterase and its associations with long-term effects of organophosphorus pesticides.

作者信息

Zhou Zhi-Jun, Zheng Jie, Wu Qiang-En, Xie Fang

机构信息

Dept. of Occupational Health and Toxicology, School of Public Health, WHO Collaborating Center for Occupational Health / Key Laboratory of Public Health and Safety, Ministry of Education, Fudan University, Shanghai 200032, China.

出版信息

Biomed Environ Sci. 2007 Aug;20(4):284-90.

PMID:17955620

Abstract

OBJECTIVE

To examine a) the effect of organophosphorus pesticide exposure on activity of carboxylic esterases, namely butyrylcholinesterase (BChE), carboxylesterase (CarbE) and paraoxonase (PonE); and b) the association of polymorphisms of BChE and PonE with individual genetic susceptibility to organophosphorus pesticide exposure.

METHODS

A cross-sectional study was conducted in 75 workers exposed to organophosphorus pesticides and 100 non-exposed controls. The serum activity of these enzymes was measured. Variant forms of BCHE-K, PON-192, and PON-55 were detected. A symptom score was developed as a proxy measure of clinical outcomes.

RESULTS

Activities of both BChE and CarbE were lower in exposed workers (27.3+/-21.65 nmolxh(-1)xmL(-1) and 235.6+/-104.03 nmolxmin(-1)xmL(-1)) than in non-exposed workers (78.313+/-30.354 nmolxh(-1)xmL(-1) and 362.681+/-194.997 nmolxmin(-1)xmL(-1)). The activity of PonE was not associated with exposure status. The AChE activity in the exposed workers with BCHE-K genotype UU (61 cases), genotype UK (12 cases) and genotype KK (2 cases) was 105.05, 84.42 and 79.00 mmolxh(-1)xmL(-1), respectively and the accumulative symptom scores were 3.74, 9.17, and 12.50 accordingly. The AChE activity in the exposed workers with PON-192 genotypeBB (37), genotype AB (27) and genotype AA (11) was 116.8, 91.2, and 72.3 mmolxh(-1)xmL(-1), respectively and the symptom scores were 2.00, 6.74, and 9.73 accordingly. The AChE activity in those with PON-55 genotype LL (70) and genotype LM (5) was 102.4 and 82.8 mmolxh(-1)xmL(-1) and the symptom scores were 4.53 and 9.20. The symptom score was the highest in individuals with abnormal homozygote for each of the three gene loci.

CONCLUSIONS

Long-term exposure to organophosphorus pesticides can inhibit BChE and CarbE activity, but exerts no inhibitory effect on PonE activity. Different genotypes of BCHE-K, PON-192, and PON-55 may be related to the severity of adverse health effects of organophosphorus pesticide exposure. Implications of potentially higher susceptibility of workers with mutant homozygotes should be evaluated to reduce health risks.

摘要

目的

a）研究有机磷农药暴露对羧酸酯酶活性的影响，即丁酰胆碱酯酶（BChE）、羧酸酯酶（CarbE）和对氧磷酶（PonE）；b）研究BChE和PonE基因多态性与个体对有机磷农药暴露的遗传易感性之间的关联。

方法

对75名有机磷农药暴露工人和100名未暴露对照进行横断面研究。测量这些酶的血清活性。检测BCHE-K、PON-192和PON-55的变异形式。制定症状评分作为临床结局的替代指标。

结果

暴露工人的BChE和CarbE活性（分别为27.3±21.65 nmol·h⁻¹·mL⁻¹和235.6±104.03 nmol·min⁻¹·mL⁻¹）低于未暴露工人（分别为78.313±30.354 nmol·h⁻¹·mL⁻¹和362.681±194.997 nmol·min⁻¹·mL⁻¹）。PonE活性与暴露状态无关。BCHE-K基因型为UU（61例）、UK（12例）和KK（2例）的暴露工人的AChE活性分别为105.05、84.42和79.00 mmol·h⁻¹·mL⁻¹，累积症状评分分别为3.74、9.17和12.50。PON-192基因型为BB（37例）、AB（27例）和AA（11例）的暴露工人的AChE活性分别为116.8、91.2和72.3 mmol·h⁻¹·mL⁻¹，症状评分分别为2.00、6.74和9.73。PON-55基因型为LL（70例）和LM（5例）的AChE活性分别为102.4和82.8 mmol·h⁻¹·mL⁻¹，症状评分分别为4.53和9.20。三个基因位点中每个位点纯合子异常的个体症状评分最高。