de Miranda O P, Silva-Carvalho M C, Ribeiro A, Portela F, Cordeiro R P, Caetano N, Vidal C F L, Figueiredo A M S
Instituto de Microbiologia Prof. Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
Clin Microbiol Infect. 2007 Dec;13(12):1165-72. doi: 10.1111/j.1469-0691.2007.01830.x. Epub 2007 Oct 23.
An increasing incidence of nosocomial infections caused by non-multiresistant methicillin-resistant Staphylococcus aureus (nMMRSA) has been reported worldwide. The present study genotyped nMMRSA isolates obtained from hospitals in two cities in Brazil. The hospital isolates displayed pulsed-field gel electrophoresis (PFGE) patterns that were similar to those of the USA100 (ST5-SCCmecII) and USA 800 (ST5-SCCmecIV) strains, which are related to the New York/Japan and paediatric clones, respectively. Carriage of SCCmecIV and the classification by multilocus sequence typing (MLST) of a representative of this PFGE pattern in clonal complex 5 (CC5) confirmed the genetic relationship of the Brazilian isolates with USA800. The USA800-related Brazilian isolates were responsible for severe nosocomial infections in compromised adults and elderly patients in Brazil. A higher growth rate, an ability to form biofilm on inert polystyrene surfaces and the presence of the egc locus may have contributed, at least in part, to the fitness of these organisms as global nosocomial pathogens.
全球范围内已报道由非多重耐药性耐甲氧西林金黄色葡萄球菌（nMMRSA）引起的医院感染发病率不断上升。本研究对从巴西两个城市的医院分离出的nMMRSA菌株进行了基因分型。医院分离株显示出的脉冲场凝胶电泳（PFGE）图谱与USA100（ST5-SCCmecII）和USA 800（ST5-SCCmecIV）菌株相似，它们分别与纽约/日本克隆株和儿科克隆株相关。SCCmecIV的携带情况以及对克隆复合体5（CC5）中这种PFGE图谱的代表菌株进行多位点序列分型（MLST）分类，证实了巴西分离株与USA800的遗传关系。与USA800相关的巴西分离株导致了巴西免疫功能低下的成年人和老年患者发生严重的医院感染。更高的生长速率、在惰性聚苯乙烯表面形成生物膜的能力以及egc基因座的存在可能至少部分有助于这些微生物作为全球医院病原体的适应性。
