Schuenck Ricardo Pinto, Nouér Simone Aranha, Winter Carolina de Oliveira, Cavalcante Fernanda Sampaio, Scotti Tatiana Dantas, Ferreira Adriana Lúcia Pires, Giambiagi-de Marval Márcia, dos Santos Kátia Regina Netto
Departamento de Microbiologia Médica, Instituto de Microbiologia Prof. Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-590, Brazil.
Diagn Microbiol Infect Dis. 2009 Aug;64(4):434-41. doi: 10.1016/j.diagmicrobio.2009.04.007.
Change in epidemiology of methicillin-resistant Staphylococcus aureus (MRSA) was observed because of the emergence of infections by non-multiresistant MRSA (nMRSA) in our hospital in Rio de Janeiro, Brazil. Clinical characterization and molecular analysis of 20 nMRSA isolates recovered from 17 patients, between February 2005 and March 2006, were performed. The analysis included SCCmec (staphylococcal cassette chromosome mec), pulsed field gel electrophoresis (PFGE), multilocus restriction fragment, and multilocus sequence typing. MICs for oxacillin and vancomycin and presence of Panton-Valentine leukocidin (PVL) genes were also investigated. All but 1 of the 20 isolates presented SCCmec type IV. PFGE clustered all isolates into 9 genotypes. MIC < or = 16 microg/mL to oxacillin was found for 65% of the isolates, whereas 80% exhibited MIC of 2 microg/mL for vancomycin. PVL-encoding genes were observed in 3 isolates. Polyclonal presence of nMRSA SCCmec IV was observed in our institution, including community and health care-associated isolates, which belonged to the sequence types (STs) 1 (clonal complex [CC1]), ST5 (CC5), ST8 and ST72 (CC8), ST97 (CC97), and 2 ST singletons (SLV5 and SLV30).
由于巴西里约热内卢我们医院出现了非多重耐药性耐甲氧西林金黄色葡萄球菌（nMRSA）感染，耐甲氧西林金黄色葡萄球菌（MRSA）的流行病学发生了变化。对2005年2月至2006年3月期间从17名患者中分离出的20株nMRSA进行了临床特征分析和分子分析。分析包括葡萄球菌盒式染色体mec（SCCmec）、脉冲场凝胶电泳（PFGE）、多位点限制性片段分析和多位点序列分型。还研究了对苯唑西林和万古霉素的最低抑菌浓度（MIC）以及杀白细胞素（PVL）基因的存在情况。20株分离株中除1株外均呈现SCCmec IV型。PFGE将所有分离株分为9种基因型。65%的分离株对苯唑西林的MIC≤16μg/mL，而80%的分离株对万古霉素的MIC为2μg/mL。在3株分离株中观察到了PVL编码基因。在我们机构中观察到nMRSA SCCmec IV的多克隆存在，包括社区和医疗保健相关分离株，它们属于序列类型（STs）1（克隆复合体[CC1]）、ST5（CC5）、ST8和ST72（CC8）、ST97（CC97）以及2个单序列类型（SLV5和SLV30）。
