Guarini Monica, Stabile Angela, Cavallini Gabriella, Donati Alessio, Bergamini Ettore
Centro di Ricerca Interdipartimentale di Biologia e Patologia dell'Invecchiamento dell'Università di Pisa, Italy.
Free Radic Res. 2007 Nov;41(11):1283-8. doi: 10.1080/10715760701689568.
Dolichol, a long-chain polyisoprenoid broadly distributed in all tissues and cellular membranes with unknown function(s), might have a role in free radical metabolism [it accumulates in older tissues and decreases after CCl4 (in liver) or phenylhydrazine (in spleen and liver) administration]. The effects of the NADPH-ADP-Fe system on Dolichol levels in isolated hepatocytes were explored and the time-course of changes was compared with the release of MDA in the incubation medium and the decrease in CoQ 9 and 10 and Vitamin E levels. Results showed that the system increased lipid peroxidation and decreased Dolichol and CoQ levels in-parallel fashions and lowered Vitamin E levels with shorter latency. Meanwhile, no increase in dead cells and no Dolichol release in the medium were detected. In conclusion, an increase in oxidative stress possibly caused a rapid degradation of dolichol by the same (unknown) mechanism responsible for the breakdown of Ubiquinone isoprenoid chains.
多萜醇是一种广泛分布于所有组织和细胞膜中的长链聚异戊二烯，其功能尚不清楚，可能在自由基代谢中发挥作用[它在较老的组织中积累，在给予四氯化碳（肝脏中）或苯肼（脾脏和肝脏中）后会减少]。研究了NADPH-ADP-铁系统对分离的肝细胞中多萜醇水平的影响，并将变化的时间进程与孵育培养基中丙二醛的释放以及辅酶Q9和10以及维生素E水平的降低进行了比较。结果表明，该系统以平行方式增加脂质过氧化，降低多萜醇和辅酶Q水平，并以较短的延迟降低维生素E水平。同时，未检测到死细胞增加，培养基中也没有多萜醇释放。总之，氧化应激的增加可能通过与负责泛醌异戊二烯链分解的相同（未知）机制导致多萜醇快速降解。
