Cavallini G, Straniero S, Donati A, Bergamini E
Sara Straniero, Department of Medicine, Center for Diabetes, Metabolism and Endocrinology, Karolinska Institute, Karolinska University Hospital, Huddinge, 141 86 Stockholm, Sweden, Tel +46 8 52481097, e-mail:
J Nutr Health Aging. 2016;20(5):540-5. doi: 10.1007/s12603-015-0611-z.
There is substantial evidence that a diet rich in fruit and vegetables may reduce the risk of aging and stress oxidative associated diseases. It has been suggested that benefits associated with fruit and red wine consumption could be due to pooled antioxidant microcomponents in diet. The aim of this study was to investigate the antioxidant activities of pure resveratrol (a well known phytoalexin, RSV) and red wine polyphenols (RWP), using UV-B radiated isolated rat hepatocytes as a model of oxidative stress.
Rat hepatocytes were isolated by the collagenase method. The cells were loaded with resveratrol and/or polyphenols at different concentrations. The production of thiobarbituric acid reactive substances (TBARS) released by UV-B radiated cells and the levels of lipid-soluble antioxidants (Dolichol, Vitamin E, Coenzyme Q9 and Q10) were measured.
Resveratrol had pro-oxidant or antioxidant effects depending on (lower or higher) dosage. RWP protection from photolipoperoxidation was dose-dependent and increased with dosage. Combination of the two compounds exhibited synergistic antioxidant effect, and made resveratrol effective both at lower and higher dosages.
These results suggest that resveratrol requires red wine polyphenols for optimum antioxidant activity.
有大量证据表明，富含水果和蔬菜的饮食可降低衰老风险以及与氧化应激相关的疾病风险。有人认为，食用水果和红酒带来的益处可能归因于饮食中混合的抗氧化微量成分。本研究的目的是以紫外线B辐射分离的大鼠肝细胞作为氧化应激模型，研究纯白藜芦醇（一种著名的植物抗毒素，RSV）和红酒多酚（RWP）的抗氧化活性。
采用胶原酶法分离大鼠肝细胞。用不同浓度的白藜芦醇和/或多酚处理细胞。测量紫外线B辐射细胞释放的硫代巴比妥酸反应性物质（TBARS）的产生量以及脂溶性抗氧化剂（多萜醇、维生素E、辅酶Q9和Q10）的水平。
白藜芦醇根据剂量（较低或较高）产生促氧化或抗氧化作用。RWP对光致脂质过氧化的保护作用呈剂量依赖性，且随剂量增加而增强。两种化合物的组合表现出协同抗氧化作用，使白藜芦醇在较低和较高剂量下均有效。
这些结果表明，白藜芦醇需要红酒多酚才能发挥最佳抗氧化活性。
