Reyes Romina C, Stoakes Luba, Milburn Sue, Lennox Gwen, Daniel Janis, Silver Shannon N, Wijnker E, John Michael A, Hussain Zafar
London Health Sciences Centre, London, Ontario, Canada.
Diagn Microbiol Infect Dis. 2008 Feb;60(2):225-7. doi: 10.1016/j.diagmicrobio.2007.09.011. Epub 2007 Oct 24.
A new chromogenic medium, denim blue (DB), was compared with methicillin-resistant Staphylococcus aureus (MRSA) Select (MRSAS) for detection of MRSA from surveillance specimens. On DB, MRSA colonies are larger (0.57-0.8 versus 0.45-0.6 mm at 18 and 24 h, respectively). Despite this, sensitivities of DB were 77% and 96% at 18 and 24 h, respectively, and those of MRSAS were 63% and 97.5%. Specificities were significantly higher for MRSAS than DB. The study demonstrates that DB and MRSA are equal for the detection of MRSA from surveillance specimens provided the plates are read after 24 h of incubation. Positive predictive values of both media were less than 95% requiring confirmation of MRSA, by another method, from all new patients.
一种新的显色培养基——牛仔蓝（DB），与耐甲氧西林金黄色葡萄球菌（MRSA）选择培养基（MRSAS）进行了比较，用于从监测标本中检测MRSA。在DB培养基上，MRSA菌落更大（18小时和24小时时分别为0.57 - 0.8毫米和0.45 - 0.6毫米）。尽管如此，DB培养基在18小时和24小时时的敏感性分别为77%和96%，而MRSAS的敏感性分别为63%和97.5%。MRSAS的特异性显著高于DB。该研究表明，如果在孵育24小时后读取平板，DB和MRSAS在从监测标本中检测MRSA方面效果相当。两种培养基的阳性预测值均低于95%，因此需要通过另一种方法对所有新患者的MRSA进行确认。
