Ben Nsira Sana, Dupuis Michel, Leclercq Roland
Service de Microbiologie, Hôpital Côte de Nacre, Université de Caen, 14033 Caen Cedex, France.
Int J Antimicrob Agents. 2006 Jun;27(6):561-4. doi: 10.1016/j.ijantimicag.2006.03.011. Epub 2006 May 11.
MRSA Select is a new chromogenic medium for the detection from clinical specimens of methicillin-resistant Staphylococcus aureus (MRSA) growing as pink colonies. Screening samples (n=666) taken from patients were spread onto MRSA Select, oxacillin resistance screening agar base (ORSAB), mannitol salt agar and an in-house medium containing tobramycin-colistin-fungizone (TCF). Ninety-nine samples were positive for MRSA on at least one selective medium. Sensitivity and specificity were >94% for all media provided that incubation was extended to 48 h for ORSAB and that a coagulase test was performed for ORSAB and TCF. By contrast, on the single criterion of the colour of colonies for MRSA positivity, the specificity and sensitivity of MRSA Select were high (99.8% and 99%, respectively).
MRSA Select是一种新型显色培养基，用于从临床标本中检测出呈粉红色菌落生长的耐甲氧西林金黄色葡萄球菌（MRSA）。将取自患者的筛查样本（n = 666）接种于MRSA Select、苯唑西林耐药性筛查琼脂基础培养基（ORSAB）、甘露醇盐琼脂和含妥布霉素-黏菌素-两性霉素B（TCF）的自制培养基上。至少在一种选择性培养基上有99份样本的MRSA呈阳性。如果ORSAB的培养时间延长至48小时，并且对ORSAB和TCF进行凝固酶试验，则所有培养基的敏感性和特异性均> 94%。相比之下，根据菌落颜色这一单一标准判断MRSA是否呈阳性，MRSA Select的特异性和敏感性很高（分别为99.8%和99%）。
