Bresler S E, Kachurin A M, Ogurtsova N I, Fomichev V N
Biokhimiia. 1976 Mar;41(3):426-31.
The study of the flavin mononucleotide (FMN)-reduced nicotinamide adeninedinucleotide (NADH) reaction was carried out both under aerobic and anaerobic conditions, using spectrophotometric and titrimetric methods. The consumption of NADH was shown to exceed two times the consumption of FMN in the anaerobic reaction and the rate constant in the aerobic reaction was found to be about 4 times of that of the anaerobic reaction. Moreover, the replacement of anaerobic conditions by aerobic ones at pH 5.0 resulted in a considerable increase of proton consumption rate in the reaction course. The data obtained are contradictory to the generally accepted hypothesis of hydrid-ion transfer in the reaction of NADH oxidation. It was assumed that this reaction proceeded through a homolytic pathway.
采用分光光度法和滴定法，在有氧和无氧条件下对黄素单核苷酸（FMN）-还原型烟酰胺腺嘌呤二核苷酸（NADH）反应进行了研究。结果表明，在厌氧反应中，NADH的消耗量超过FMN消耗量的两倍，且有氧反应中的速率常数约为厌氧反应的4倍。此外，在pH 5.0时将厌氧条件替换为有氧条件，导致反应过程中质子消耗速率显著增加。所得数据与NADH氧化反应中普遍接受的氢负离子转移假说相矛盾。据推测，该反应通过均裂途径进行。
