Bresler S E, Isaeva-Ivanova L S, Ogurtsova N I, Fomichev V N
Biokhimiia. 1976 Apr;41(4):579-84.
The reduction of flavin by reduced diphosphopyridine nucleotide and photoreduction were studied spectrophotometrically. Flavin was covalently bound to aminoethylcellulose, therefore the interaction between flavin molecules was excluded. Nevertheless a considerable quantity of free radicals was demonstrated under these conditions. The experimental dependence of the radical concentration as a function of reduction degree is readily explained if the reduction of flavin proceeds in two consecutive one-electron steps. The ratio of the rate constants of both reactions for the reduction of flavin by NADH k2'/k1' was equal to 4, for the photoreductions k2'/k1' to 6.5.
用分光光度法研究了黄素被还原型二磷酸吡啶核苷酸还原以及光还原的过程。黄素与氨乙基纤维素共价结合，因此排除了黄素分子之间的相互作用。然而，在这些条件下仍证明存在大量自由基。如果黄素的还原以两个连续的单电子步骤进行，那么自由基浓度作为还原程度函数的实验依赖性很容易解释。对于由NADH还原黄素的反应，两个反应的速率常数之比k2'/k1'等于4，对于光还原反应，k2'/k1'等于6.5。
