Muro A, Cordero M, Ramos A, Simón F
Laboratorie de Parasitología, Facultad de Farmacia, Universidad de Salmanca, Spain.
Trop Med Parasitol. 1991 Dec;42(4):371-4.
A prospective follow-up study to define seasonal changes in the level of anti-Dirofilaria immitis antibodies in an exposed human population in Western Spain was conducted. Specific antibodies were detected using an enzymelinked immunosorbent assay (ELISA). Ig M antibodies predominated in Summer, during maximal vector activity, while Ig G rose to a maximum in Winter. The sole risk factor associated with higher optical densities in the ELISA proved to be age, indicating that repeated exposure to the parasite is the rule. It is concluded that, for serodiagnosis the endemic or non-endemic nature of the geographical area and the season of the year should be considered to define the basal level of positivity, and that as well as Ig G, Ig M should always be determined.
在西班牙西部一个受暴露的人群中开展了一项前瞻性随访研究，以确定抗犬恶丝虫抗体水平的季节性变化。使用酶联免疫吸附测定（ELISA）检测特异性抗体。IgM抗体在夏季媒介活动高峰期占主导，而IgG在冬季升至最高。ELISA中与较高光密度相关的唯一风险因素是年龄，这表明反复接触该寄生虫是常见情况。得出的结论是，对于血清诊断，应考虑地理区域的地方病或非地方病性质以及一年中的季节，以确定阳性的基础水平，并且除了IgG之外，还应始终测定IgM。
