Sarrafzadeh Mohammad H, Guiraud Joseph P, Lagneau Christophe, Gaven Bruno, Carron Alexandre, Navarro Jean-Marie
UMR Ingénierie des Réactions Biologiques, Bioproductions, Polytech, Université Montpellier II, Montpellier 34095, France.
Curr Microbiol. 2005 Aug;51(2):75-81. doi: 10.1007/s00284-005-4463-3. Epub 2005 Jul 13.
Growth, sporulation, synthesis of delta-endotoxins, and toxicity against the larvae of Aedes aegypti and Culex pipiens were studied during fermentation of Bacillus thuringiensis H14 in a 20-L fermentor. Measurements of optical density and dielectric permittivity for biomass determination suggest a highly promising technique for on-line evaluation of sporulation. The synthesis of 65-, 25- and 130-kDa proteins started at 16, 18, and 23 h, respectively. These proteins were enriched in different ways until the end of culture (48 h). Toxicity in the course of sporulation was significantly different for the larvae of both mosquito species. Maximal activity against Ae. aegypti was obtained at the end of culture, whereas for Cx. pipiens, the sample at 38 h was the most active.
在20升发酵罐中对苏云金芽孢杆菌H14进行发酵时，研究了其生长、芽孢形成、δ-内毒素的合成以及对埃及伊蚊和致倦库蚊幼虫的毒性。通过测量光密度和介电常数来测定生物量，这表明了一种极有前景的在线评估芽孢形成的技术。65 kDa、25 kDa和130 kDa蛋白质的合成分别在16小时、18小时和23小时开始。这些蛋白质以不同方式富集直至培养结束（48小时）。在芽孢形成过程中，两种蚊虫幼虫的毒性存在显著差异。对埃及伊蚊的最大活性在培养结束时获得，而对于致倦库蚊，38小时的样品活性最高。
