Laerum O D, Hülser D F, Rajewsky M F
Cancer Res. 1976 Jul;36(7 PT 1):2153-61.
A comparative analysis was performed on the electrophysiological properties of 11 neoplastic neurogenic cell culture lines and five other cell lines of different origin (HV1C, rat bile duct carcinoma; BICR/M1RK, rat mammary tumor; HeLa, human cervix carcinoma; 3T3, mouse embryo; REe, rat embryo). Neurogenic lines were derived either from N-ethyl-N-nitrosourea-induced neoplasms of the nervous system or from cultured fetal rat brain cells that had undergone neoplastic transformation in vitro after exposure to N-ethyl-N-nitrosourea in vivo. Electrical membrane excitability was lacking in all neurogenic cells analyzed. Their membrane potential and input resistance values were similar to those of the nonneurogenic lines. Intercellular ionic coupling was consistently observed between cells of a fibroblastoid shape or cells bearing multiple cytoplasmic processes (i.e., all neurogenic lines HV1C, BICR/M1RK, and 3T3). Epithelioid cells (i.e., HeLa, REe, an NV1C subpopulation, and a GV1C1 variant) showed no such intercellular communication. In vivo monolayer cultures on glass coverslips were obtained by a modified i.p. diffusion chamber technique. Under these conditions, the cells (with the exception of a glioma-derived cell line) retained the morphological appearance and electrophysiological properties observed in vitro.
对11种肿瘤性神经源性细胞系和其他5种不同来源的细胞系（HV1C，大鼠胆管癌；BICR/M1RK，大鼠乳腺肿瘤；HeLa，人宫颈癌；3T3，小鼠胚胎；REe，大鼠胚胎）的电生理特性进行了比较分析。神经源性细胞系要么源自N-乙基-N-亚硝基脲诱导的神经系统肿瘤，要么源自经体内暴露于N-乙基-N-亚硝基脲后在体外发生肿瘤转化的培养大鼠胎脑细胞。在所分析的所有神经源性细胞中均缺乏膜电兴奋性。它们的膜电位和输入电阻值与非神经源性细胞系相似。在成纤维细胞样形状的细胞或带有多个细胞质突起的细胞（即所有神经源性细胞系HV1C、BICR/M1RK和3T3）之间始终观察到细胞间离子偶联。上皮样细胞（即HeLa、REe、NV1C亚群和GV1C1变体）未显示出这种细胞间通讯。通过改良的腹腔扩散室技术在玻璃盖玻片上获得体内单层培养物。在这些条件下，细胞（除一种源自胶质瘤的细胞系外）保留了在体外观察到的形态外观和电生理特性。
