Potapova T V, Veselý P
Folia Biol (Praha). 1981;27(2):91-5.
Clonal lines of Lewis rat neoplastic fibroblastoid LW13K2 cells and epithelioid RsK4 cells were used to study the changes in membrane potential, input resistance and the capability to form low-resistance junctions upon RSV transformation. The drop of MP in RsK4 cells was accompanied by the acquisition of temperature dependence. The input resistance was simultaneously increased. This was considered to be a consequence of the decrease in potassium current across the cell membrane and of a change in the mechanism of potassium transport which became energy dependent. Examination of fluorescent tracer (n.w. 330) diffusion which was negative in the homotypic situation in both cell lines confirmed only the relative independence of these cells in culture. In the heterotypic situation LW13K2 in contact with normal mouse embryo fibroblasts gave positive results whereas RsK4 were almost negative.
采用Lewis大鼠肿瘤性成纤维样LW13K2细胞和上皮样RsK4细胞的克隆系，研究劳斯肉瘤病毒（RSV）转化后细胞膜电位、输入电阻及形成低电阻连接能力的变化。RsK4细胞中膜电位的下降伴随着温度依赖性的获得。同时输入电阻增加。这被认为是细胞膜钾电流减少以及钾转运机制改变（变为能量依赖性）的结果。对荧光示踪剂（分子量330）扩散的检测在两种细胞系的同型情况下均为阴性，这仅证实了这些细胞在培养中的相对独立性。在异型情况下，与正常小鼠胚胎成纤维细胞接触的LW13K2产生阳性结果，而RsK4几乎为阴性。
