结直肠癌中紧密连接蛋白-1和紧密连接蛋白-2的选择性上调
Selective up-regulation of claudin-1 and claudin-2 in colorectal cancer.
作者信息
Kinugasa Tetsushi, Huo Qun, Higashi Daijiro, Shibaguchi Hirotomo, Kuroki Motomu, Tanaka Toshihiro, Futami Kitarou, Yamashita Yuichi, Hachimine Ken, Maekawa Shinichi, Nabeshima Kazuki, Iwasaki Hiroshi, Kuroki Masahide
机构信息
Department of Biochemistry, Faculty of Medicine, Fukuoka University, Fukuoka, Japan.
出版信息
Anticancer Res. 2007 Nov-Dec;27(6A):3729-34.
BACKGROUND
To understand the molecular and morphological alterations in the tight junction in colorectal cancer (CRC) tissues, the expression of eight tight junction proteins in normal and cancer colorectal tissues were compared.
PATIENTS AND METHODS
Adenocarcinoma tissues and paired normal mucosa were resected from surgical specimens of CRC patients. The expression of occludin, ZO-1, ZO-2, and claudin-1 -5 was analyzed at the mRNA level by quantitative reverse transcription-polymerase chain reaction (RT-PCR) and at the protein level by immunohistochemistry.
RESULTS
The expression of claudin-1 and claudin-2 in cancer tissues was upregulated 40- and 49.2-fold, respectively, at the mRNA level, as compared with that in normal tissues. The up-regulation of these two claudins was also observed at the protein level and it appeared to depend on the depth of tumor invasion.
CONCLUSION
Claudin-1 and claudin-2 were found to be overexpressed in CRC tissues. They may be useful as tumor markers and targets for the treatment of colorectal cancer.
背景
为了解结直肠癌（CRC）组织紧密连接中的分子和形态学改变，比较了正常和癌性结直肠组织中8种紧密连接蛋白的表达。
患者和方法
从CRC患者的手术标本中切除腺癌组织和配对的正常黏膜。通过定量逆转录-聚合酶链反应（RT-PCR）在mRNA水平以及通过免疫组织化学在蛋白质水平分析闭合蛋白、紧密连接蛋白1（ZO-1）、紧密连接蛋白2（ZO-2）和闭合蛋白1-5的表达。
结果
与正常组织相比，癌组织中闭合蛋白1和闭合蛋白2在mRNA水平的表达分别上调了40倍和49.2倍。在蛋白质水平也观察到这两种闭合蛋白的上调，并且其似乎取决于肿瘤浸润深度。
结论
发现闭合蛋白1和闭合蛋白2在CRC组织中过表达。它们可能作为肿瘤标志物和结直肠癌治疗的靶点。
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