Monciardini P, Montanini N, Sosio M, Donadio S
KtedoGen, Milano, Italy.
Lett Appl Microbiol. 2008 Jan;46(1):87-94. doi: 10.1111/j.1472-765X.2007.02271.x. Epub 2007 Oct 27.
Efficient strain dereplication is of great value during the generation of bacterial strain collections for industrial screening. We evaluated the utilization of the RNase P RNA gene (rnpB) sequence as a tool for molecular dereplication of myxobacteria.
16S rDNA (approx. 1 x 5 kbp) and rnpB (approx. 0 x 3 kbp) sequences were obtained and aligned. From 50 strains, we obtained 20 different sequences for the 16S rDNA and 24 for rnpB. Intersequence similarity was lower for rnpB than for 16S rDNA.
rnpB allows the rapid discrimination of similar strains, with a higher resolution power as compared with 16S rRNA gene sequencing. It not only gives better discrimination, but is also faster and cheaper than 16S rDNA sequencing.
Myxobacteria isolation and cultivation require time and experience. The application of rnpB sequencing to early myxobacterial strain dereplication may help in the generation of diverse strain libraries of these bacteria.
在构建用于工业筛选的细菌菌株库过程中，高效的菌株重复排除具有重要价值。我们评估了核糖核酸酶P RNA基因（rnpB）序列作为黏细菌分子重复排除工具的实用性。
获取并比对了16S rDNA（约1×5 kbp）和rnpB（约0×3 kbp）序列。从50个菌株中，我们获得了20种不同的16S rDNA序列和24种rnpB序列。rnpB序列间的相似性低于16S rDNA。
与16S rRNA基因测序相比，rnpB能够快速区分相似菌株，具有更高的分辨能力。它不仅能提供更好的区分效果，而且比16S rDNA测序更快、更便宜。
黏细菌的分离和培养需要时间和经验。将rnpB测序应用于早期黏细菌菌株的重复排除，可能有助于构建这些细菌的多样菌株文库。
