Hyun Hyesook, Chung Jinwoo, Kim Jihoon, Lee Jong Suk, Kwon Byoung-Mog, Son Kwang-Hee, Cho Kyungyun
Myxobacteria Bank, Department of Biotechnology, Hoseo University, Asan 336-795, Korea.
J Microbiol Biotechnol. 2008 Aug;18(8):1416-22.
Epothilone and its analogs are a potent new class of anticancer compounds produced by myxobacteria. Thus, in an effort to identify new myxobacterial strains producing epothilone and its analogs, cellulose-degrading myxobacteria were isolated from Korean soils, and 13 strains carrying epothilone biosynthetic gene homologs were screened using a polymerase chain reaction. A migration assay revealed that Sorangium cellulosum KYC3013, 3016, 3017, and 3018 all produced microtubule-stabilizing compounds, and an LCMS/ MS analysis showed that S. cellulosum KYC3013 synthesized epothilone A.
埃坡霉素及其类似物是由粘细菌产生的一类强效新型抗癌化合物。因此，为了鉴定产生埃坡霉素及其类似物的新粘细菌菌株，从韩国土壤中分离出纤维素降解粘细菌，并使用聚合酶链反应筛选出13株携带埃坡霉素生物合成基因同源物的菌株。迁移试验表明，纤维堆囊菌KYC3013、3016、3017和3018均产生微管稳定化合物，液相色谱-质谱联用/质谱分析表明，纤维堆囊菌KYC3013合成了埃坡霉素A。
