Jiang De-Ming, Zhao Lin, Zhang Cui-Ying, Li Jian, Xia Zhi-Jie, Wang Jing, Wu Zhi-Hong, Li Yue-Zhong
State Key Laboratory of Microbial Technology, College of Life Science, Shandong University, Jinan 250100, PR China.
Int J Syst Evol Microbiol. 2008 Nov;58(Pt 11):2654-9. doi: 10.1099/ijs.0.65806-0.
The taxonomy of myxobacteria is based mainly on their morphological characteristics. The genus Sorangium belongs to the myxobacterial suborder Sorangiineae. Strains in the genus were classified either as one species, Sorangium cellulosum, by ignoring divergent morphological characteristics, or into several species; however, the latter classification is based on some dubious morphological characteristics and is inconsistent with the phylogeny constructed from 16S rRNA gene sequences. In this study, two HSP60 (groEL1 and groEL2) genes were amplified and sequenced from 22 Sorangium strains. The groEL1 and groEL2 gene sequences were highly conserved in Sorangium strains, suggesting that these two paralogous genes both play important roles in the life cycle. The phylogeny constructed by the groEL genes was rather consistent with the morphological characteristics of sporangioles. Including information from the phylogenetic analysis and morphological characteristics, it is suggested that the genus Sorangium includes two species.
粘细菌的分类主要基于其形态特征。堆囊菌属属于粘细菌亚目堆囊菌亚目。该属中的菌株要么被归为一个物种，即纤维素堆囊菌，而忽略了不同的形态特征，要么被分为几个物种；然而，后一种分类是基于一些可疑的形态特征，并且与根据16S rRNA基因序列构建的系统发育不一致。在本研究中，从22株堆囊菌菌株中扩增并测序了两个HSP60（groEL1和groEL2）基因。groEL1和groEL2基因序列在堆囊菌菌株中高度保守，表明这两个旁系同源基因在生命周期中都发挥着重要作用。由groEL基因构建的系统发育与小孢囊的形态特征相当一致。综合系统发育分析和形态特征的信息，表明堆囊菌属包括两个物种。
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