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使用体外龈下生物膜模型研究功能化聚合物纳米颗粒对钛表面的抗菌作用。

Antibacterial Effect of Functionalized Polymeric Nanoparticles on Titanium Surfaces Using an In Vitro Subgingival Biofilm Model.

作者信息

Bueno Jaime, Virto Leire, Toledano-Osorio Manuel, Figuero Elena, Toledano Manuel, Medina-Castillo Antonio L, Osorio Raquel, Sanz Mariano, Herrera David

机构信息

ETEP (Etiology and Therapy of Periodontal and Peri-Implant Diseases) Research Group, University Complutense, Pza. Ramón y Cajal s/n, 28040 Madrid, Spain.

Faculty of Dentistry, University of Granada, Colegio Máximo de Cartuja s/n, 18071 Granada, Spain.

出版信息

Polymers (Basel). 2022 Jan 18;14(3):358. doi: 10.3390/polym14030358.

DOI:10.3390/polym14030358
PMID:35160348
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8839475/
Abstract

This investigation aimed to evaluate the antibacterial effect of polymeric nanoparticles (NPs), functionalized with calcium, zinc, or doxycycline, using a subgingival biofilm model of six bacterial species ( and ) on sandblasted, large grit, acid-etched titanium discs (TiDs). Undoped NPs (Un-NPs) or doped NPs with calcium (Ca-NPs), zinc (Zn-NPs), or doxycycline (Dox-NPs) were applied onto the TiD surfaces. Uncovered TiDs were used as negative controls. Discs were incubated under anaerobic conditions for 12, 24, 48, and 72 h. The obtained biofilm structure was studied by scanning electron microscopy (SEM) and its vitality and thickness by confocal laser scanning microscopy (CLSM). Quantitative polymerase chain reaction of samples was used to evaluate the bacterial load. Data were evaluated by analysis of variance ( < 0.05) and post hoc comparisons with Bonferroni adjustments ( < 0.01). As compared with uncovered TiDs, Dox-NPs induced higher biofilm mortality (47.21% and 85.87%, respectively) and reduced the bacterial load of the tested species, after 72 h. With SEM, scarce biofilm formation was observed in Dox-NPs TiDs. In summary, Dox-NPs on TiD reduced biofilm vitality, bacterial load, and altered biofilm formation dynamics.

摘要

本研究旨在使用六种细菌的龈下生物膜模型，对喷砂、大颗粒、酸蚀钛盘（TiD）评估用钙、锌或强力霉素功能化的聚合物纳米颗粒（NP）的抗菌效果。将未掺杂的NP（Un-NP）或掺杂钙（Ca-NP）、锌（Zn-NP）或强力霉素（Dox-NP）的NP应用于TiD表面。未覆盖的TiD用作阴性对照。将圆盘在厌氧条件下孵育12、24、48和72小时。通过扫描电子显微镜（SEM）研究获得的生物膜结构，并通过共聚焦激光扫描显微镜（CLSM）研究其活力和厚度。使用样品的定量聚合酶链反应来评估细菌载量。通过方差分析（<0.05）和采用Bonferroni校正的事后比较（<0.01）来评估数据。与未覆盖的TiD相比，72小时后，Dox-NP诱导更高的生物膜死亡率（分别为47.21%和85.87%），并降低了受试菌种的细菌载量。通过SEM观察到，Dox-NP TiD上生物膜形成稀少。总之，TiD上的Dox-NP降低了生物膜活力、细菌载量，并改变了生物膜形成动态。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e343/8839475/7c40fe8c0f5b/polymers-14-00358-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e343/8839475/77e344e424c3/polymers-14-00358-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e343/8839475/fe2b046eabbc/polymers-14-00358-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e343/8839475/78d4d8f829cb/polymers-14-00358-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e343/8839475/0e4799bc20bd/polymers-14-00358-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e343/8839475/7c40fe8c0f5b/polymers-14-00358-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e343/8839475/77e344e424c3/polymers-14-00358-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e343/8839475/fe2b046eabbc/polymers-14-00358-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e343/8839475/78d4d8f829cb/polymers-14-00358-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e343/8839475/0e4799bc20bd/polymers-14-00358-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e343/8839475/7c40fe8c0f5b/polymers-14-00358-g005.jpg

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