Tg(9 HSA-MYC)，一种在小鼠中纯合致死的插入。
Tg (9 HSA-MYC), a homozygous lethal insertion in the mouse.
作者信息
Tutois S, Salaun J, Mattei M G, Guénet J L
机构信息
Unité de Génétique des Mammifères, Institut Pasteur, Paris, France.
出版信息
Mamm Genome. 1991;1(3):184-90. doi: 10.1007/BF00351065.
Transgenic mice generated with different DNA sequences were surveyed for possible homozygous mutant phenotypes. We found an embryonic lethal mutation in the transgenic mouse strain (MT-MYC12.4) containing the human c-myc gene. Embryos homozygous for the transgene die shortly after implantation. The strain MT-MYC12.4 carries approximately 50 tandem copies of the recombinant plasmid sequence. The 3' flanking sequence has been cloned and analyzed. It contains a unique sequence that has been conserved during evolution and maps to Chromosome (Chr) 9. This mutant has been designated Tg 9 (HSA-MYC).
对用不同DNA序列产生的转基因小鼠进行了调查，以寻找可能的纯合突变体表型。我们在含有人类c-myc基因的转基因小鼠品系（MT-MYC12.4）中发现了一个胚胎致死突变。转基因纯合的胚胎在着床后不久死亡。MT-MYC12.4品系携带约50个串联的重组质粒序列拷贝。其3'侧翼序列已被克隆和分析。它包含一个在进化过程中保守且定位于9号染色体（Chr）的独特序列。这个突变体已被命名为Tg 9（HSA-MYC）。
Zotero 插件