Constantin J, Ishii-Iwamoto E L, Ferraresi-Filho O, Kelmer-Bracht A M, Bracht A
Departamento de Bioquímica, Universidade de Maringá, Brasil.
Braz J Med Biol Res. 1991;24(8):767-71.
The effect of stevioside, an inhibitor of long-chain fatty acid transport, on ketogenesis and on [14C]CO2 production from [1-14C]palmitate (100-300 microM) was investigated in the isolated and hemoglobin-free perfused rat liver. Stevioside (2.5 mM), a sweet glycoside found in Stevia rebaudiana leaves, inhibited both parameters, but had a lower effect on [14C]CO2 production. At 300 microM palmitate and 150 microM albumin, for example, ketogenesis was inhibited by 66.3%, whereas no significant inhibition of [14C]CO2 was demonstrable. These results were interpreted to reflect 1) different degrees of saturation of the citric acid cycle and the ketogenic pathway and 2) changes in the redox state of the mitochondrial NAD(+)-NADH couple which may also occur upon stevioside infusion.
在分离且无血红蛋白灌注的大鼠肝脏中，研究了长链脂肪酸转运抑制剂甜菊糖苷对生酮作用以及[1-14C]棕榈酸酯（100 - 300微摩尔）生成[14C]CO2的影响。甜菊糖苷（2.5毫摩尔）是一种从甜叶菊叶片中发现的甜味糖苷，它抑制了这两个参数，但对[14C]CO2生成的影响较小。例如，在300微摩尔棕榈酸酯和150微摩尔白蛋白存在时，生酮作用被抑制了66.3%，而[14C]CO2没有明显受到抑制。这些结果被解释为反映了：1）柠檬酸循环和生酮途径不同程度的饱和度；2）线粒体NAD(+)-NADH偶联的氧化还原状态变化，这种变化在输注甜菊糖苷时也可能发生。
