Perumal Shanmugapriya, Mahmud Roziahanim, Mohamed Nornisah
School of Pharmaceutical Sciences, Universiti Sains Malaysia, 11800 Penang, Malaysia.
Evid Based Complement Alternat Med. 2018 Jul 11;2018:5713703. doi: 10.1155/2018/5713703. eCollection 2018.
is naturally resistant to many classes of antipseudomonal antibiotics due to the species ability to easily acquire resistance. Plant-based antibacterial agent in combination with the existing antibiotic proposes an alternative treatment regimen for the eradication of resistant bacterial infections. The antibacterial effects of the isolated epicatechin 3-gallate compound from in combination with cefepime were investigated against resistant . The fractional inhibitory concentration index of the combination was determined using checkerboard broth microdilution method. Epicatechin 3-gallate combined with cefepime had produced synergistic effect against (with average FIC index of 0.24). The MIC of epicatechin 3-gallate was effectively reduced to MIC/4, MIC/8, MIC/16, and MIC/32 in the presence of cefepime. Time-kill study of epicatechin 3-gallate combined with cefepime exhibited remarkable bactericidal activity where the eradication of occurred within 4 h of treatment. Scanning electron micrographs revealed apparent cell membrane damage and leakage of cytoplasmic contents from cells which eventually led to the cell lysis after the combination treatment of epicatechin 3-gallate and cefepime. The potential of epicatechin 3-gallate to act synergistically with cefepime against clinically resistant strain possibly will maximize the successful outcomes when choosing empirical antibiotic treatment in hospitals or health care institutions.
由于该菌种易于获得耐药性，它对多种抗假单胞菌抗生素天然耐药。植物源抗菌剂与现有抗生素联合使用，为根除耐药细菌感染提出了一种替代治疗方案。研究了从[具体来源未给出]中分离出的表儿茶素3 - 没食子酸酯化合物与头孢吡肟联合使用对耐药[细菌名称未给出]的抗菌效果。采用棋盘微量肉汤稀释法测定联合用药的部分抑菌浓度指数。表儿茶素3 - 没食子酸酯与头孢吡肟联合使用对[细菌名称未给出]产生了协同作用（平均FIC指数为0.24）。在头孢吡肟存在的情况下，表儿茶素3 - 没食子酸酯的最低抑菌浓度有效地降低到了MIC/4、MIC/8、MIC/16和MIC/32。表儿茶素3 - 没食子酸酯与头孢吡肟联合使用的时间杀菌研究显示出显著的杀菌活性，在治疗4小时内就实现了对[细菌名称未给出]的根除。扫描电子显微镜照片显示，表儿茶素3 - 没食子酸酯和头孢吡肟联合处理后，[细菌名称未给出]细胞的细胞膜明显受损，细胞质内容物泄漏，最终导致细胞裂解。表儿茶素3 - 没食子酸酯与头孢吡肟协同作用对抗临床耐药[细菌名称未给出]菌株的潜力，可能会在医院或医疗机构选择经验性抗生素治疗时使成功治疗结果最大化。
