Lee Choong-Koo, Lee Byoung-Moo, Cho Jae-Yong
Division of Animal Science and Biotechnology, Sangji University, Wonju-Si, Gangwon-Do, Korea.
Biotechnol Lett. 2008 Mar;30(3):521-7. doi: 10.1007/s10529-007-9571-7. Epub 2007 Nov 1.
The Xanthomonas oryzae pathovar oryzae gum gene cluster is composed of 14 ORFs designated, in sequence, as gumB to M, XOO3167, and gumN. The gum gene cluster constitutes an operon expressed from multiple promoters located upstream of gumB and gumG, respectively. To identify new promoters responsible for the expression of the gum gene cluster, we have conducted a computer-assisted promoter search and identified previously unreported promoter-like sequences upstream of the gumH and gumM genes, respectively. Moreover, the ability of these putative promoters to stimulate the transcription of the downstream genes was demonstrated by RT-PCR analyses using the mutant strains carrying an insertion of the rrnB transcriptional terminator into the gumG, gumH, and gumL gene, respectively.
水稻白叶枯病菌致病变种的 gum 基因簇由 14 个开放阅读框（ORF）组成，依次命名为 gumB 至 M、XOO3167 和 gumN。gum 基因簇构成一个操纵子，分别从位于 gumB 和 gumG 上游的多个启动子进行表达。为了鉴定负责 gum 基因簇表达的新启动子，我们进行了计算机辅助启动子搜索，并分别在 gumH 和 gumM 基因上游鉴定出了先前未报道的类似启动子的序列。此外，通过使用分别在 gumG、gumH 和 gumL 基因中插入了 rrnB 转录终止子的突变菌株进行 RT-PCR 分析，证明了这些推定启动子刺激下游基因转录的能力。
