Katzen F, Becker A, Zorreguieta A, Pühler A, Ielpi L
Instituto de Investigaciones Bioquímicas Fundación Campomar, Facultad de Ciencias Exactas y Naturales, Buenos Aires, Argentina.
J Bacteriol. 1996 Jul;178(14):4313-8. doi: 10.1128/jb.178.14.4313-4318.1996.
The Xanthomonas campestris gum gene cluster is composed of 12 genes designated gumB, -C, -D, -E, -F, -G, -H, -I, -J, -K, -L, and -M. The transcriptional organization of this gene cluster was analyzed by the construction of gum-lacZ transcriptional fusions in association with plasmid integration mutagenesis. This analysis, coupled with primer extension assays, indicated that the gum region was mainly expressed as an operon from a promoter located upstream of the first gene, gumB.
野油菜黄单胞菌胶基因簇由12个基因组成,分别命名为gumB、-C、-D、-E、-F、-G、-H、-I、-J、-K、-L和-M。通过构建与质粒整合诱变相关的gum-lacZ转录融合体,分析了该基因簇的转录组织。该分析与引物延伸试验相结合,表明gum区域主要从第一个基因gumB上游的启动子作为一个操纵子进行表达。