Yoon Ki-Hoon, Cho Jae-Yong
Division of Animal Science and Biotechnology, Sangji University, Wonju-si, Gangwon-do, Korea.
Biotechnol Lett. 2007 Jan;29(1):95-103. doi: 10.1007/s10529-006-9217-1. Epub 2006 Nov 8.
Genome sequence analysis of Xanthomonas oryzae pv. oryzae KACC10331 provides insight into the X. oryzae gum gene cluster that is composed of 14 open-reading frames (ORFs), designated gumB, -C, -D, -E, -F, -G, -H, -I, -J, -K, -L, -M, XOO3167, and -N. We analyzed the transcriptional linkage of the X. oryzae gum gene cluster by using RT-PCR. Analyses of the gum gene cluster by RT-PCR with the wild-type and mutant strains, which carried a deletion of the promoter-like region upstream of gumB or an insertion of the rrnB transcriptional terminator into the gumF gene, revealed that the ORFs of this gene cluster were transcribed as polycistronic mRNA, from gumB to gumN, and the secondary promoter was located upstream of gumG. Taken together, these results suggest that the genes of this cluster constitute an operon expressed from overlapping transcripts.
水稻白叶枯病菌株KACC10331的基因组序列分析，为深入了解水稻白叶枯病菌的 gum 基因簇提供了线索。该基因簇由14个开放阅读框（ORF）组成，分别命名为 gumB、-C、-D、-E、-F、-G、-H、-I、-J、-K、-L、-M、XOO3167和 -N。我们利用逆转录聚合酶链反应（RT-PCR）分析了水稻白叶枯病菌 gum 基因簇的转录连锁关系。通过对野生型和突变型菌株进行RT-PCR分析，突变型菌株缺失了 gumB 上游的类启动子区域，或者在 gumF 基因中插入了rrnB转录终止子，结果显示该基因簇的ORF以多顺反子mRNA的形式从 gumB 转录到 gumN，并且二级启动子位于 gumG 的上游。综合这些结果表明，该基因簇的基因构成了一个由重叠转录本表达的操纵子。
