El-Sayed Ghada Mohamed, Mohamed Waleed Seif El-din, Nouh Mohamed Akram, Moneer Manar Mohamed, El-Mahallawy Hadir Ahmed
Department of Clinical Pathology, National Cancer Institute, Cairo University, Cairo, Egypt.
Egypt J Immunol. 2006;13(1):105-14.
Several studies have suggested an association between Hepatitis C and B viruses (HCV and HBV) and non-Hodgkin's lymphoma (NHL). In the present study we have searched for viral genomes and antigens in the malignant lymphoma tissues as well as their seroprevalence. Antibodies against Hepatitis C as well as HCV RNA and hepatitis B surface antigen (HBsAg) were determined for 29 newly diagnosed non-Hodgkin's lymphoma patients using an enzyme linked immunosorbent assay (ELISA), as well as RT-PCR and compared with 36 apparently healthy individuals as a control group for viral markers. Immunohistochemical staining (IHC) was performed on paraffin embedded tissues for the NS3 of HCV and for HBsAg of HBV using the immunoperoxidase technique. Paraffin embedded lymph nodes (LN) were studied for the presence of viral sequences. Ten non-metastatic lymph nodes (LN) from cancer cases other than NHL were used as a control for IHC and molecular studies. HCV was significantly more encountered in patients with NHL when compared to controls for both antibodies (27.6% versus 8.3% of serum controls; p = 0.04), and antigens studied by IHC in the involved LN (41% versus 10% of tissue controls; p = 0.06). Although HBsAg positivity was not different in NHL patients when compared to controls (6.9% and 2.7%); yet it was significantly more encountered in LN of NHL patients (p = 0.04). HBV-DNA was detected in 27.5% of patient's samples and none of the controls. In conclusion, overall our findings confirm the presence of HBV and HCV antigens and viral sequences in the involved LNs of NHL patients, except for HCV RNA which perhaps necessitates fresh and not paraffin embedded tissues. These results strengthen the assumption that these viruses may be involved in the development of NHL.
多项研究表明丙型肝炎病毒（HCV）和乙型肝炎病毒（HBV）与非霍奇金淋巴瘤（NHL）之间存在关联。在本研究中，我们在恶性淋巴瘤组织中寻找病毒基因组和抗原以及它们的血清流行率。采用酶联免疫吸附测定（ELISA）以及逆转录聚合酶链反应（RT-PCR），对29例新诊断的非霍奇金淋巴瘤患者检测抗丙型肝炎抗体、HCV RNA以及乙型肝炎表面抗原（HBsAg），并与36名明显健康的个体作为病毒标志物的对照组进行比较。使用免疫过氧化物酶技术对石蜡包埋组织进行免疫组织化学染色（IHC），检测HCV的NS3和HBV的HBsAg。研究石蜡包埋淋巴结（LN）中病毒序列的存在情况。将10个非NHL癌症病例的非转移性淋巴结用作IHC和分子研究的对照。与对照组相比，NHL患者中HCV抗体（血清对照组分别为27.6%和8.3%；p = 0.04）以及通过IHC在受累LN中研究的抗原（组织对照组分别为41%和10%；p = 0.06）的检出率显著更高。尽管与对照组相比，NHL患者中HBsAg阳性率无差异（分别为6.9%和2.7%）；但在NHL患者的LN中检出率显著更高（p = 0.04）。在27.5%的患者样本中检测到HBV-DNA，而对照组均未检测到。总之，总体而言我们的研究结果证实NHL患者受累LN中存在HBV和HCV抗原及病毒序列，但HCV RNA可能需要新鲜而非石蜡包埋组织。这些结果强化了这些病毒可能参与NHL发生发展的假设。
