Desjardins Adrien E, Vakoc Benjamin J, Tearney Guillermo J, Bouma Brett E
Harvard Medical School and Wellman Center for Photomedicine, Massachusetts General Hospital, Boston 02114, USA.
Opt Lett. 2007 Nov 1;32(21):3158-60. doi: 10.1364/ol.32.003158.
Backscattering spectroscopic contrast using angle-resolved optical coherence tomography is demonstrated as a powerful method for determining scatterer diameter with subwavelength resolution. By applying spectroscopic digital processing algorithms to interferograms acquired in the frequency domain with a wavelength-swept laser centered at 1295 nm, it was shown that differences in wavelength-dependent backscattering from 0.3 and 1 microm diameter microspheres can be clearly resolved. The observed backscattering spectra were found to be consistent with Mie theory. High levels of speckle noise reduction achieved by angular compounding increased the spatial resolution at which backscattering spectra could be accurately differentiated.
利用角分辨光学相干断层扫描的背向散射光谱对比度被证明是一种用于以亚波长分辨率确定散射体直径的强大方法。通过将光谱数字处理算法应用于在频域中使用中心波长为1295 nm的扫频激光器采集的干涉图,结果表明,可以清晰分辨直径为0.3微米和1微米的微球在波长相关背向散射方面的差异。观察到的背向散射光谱与米氏理论一致。通过角度合成实现的高程度散斑噪声降低提高了能够准确区分背向散射光谱的空间分辨率。