Paramastri Yasmina, Royo Felix, Eberova Jitka, Carlsson Hans-Erik, Sajuthi Dondin, Fernstrom Anna-Linnea, Pamungkas Joka, Hau Jann
Department of Neuroscience, Division of Comparative Medicine, Uppsala University, Uppsala, Sweden.
J Med Primatol. 2007 Dec;36(6):355-64. doi: 10.1111/j.1600-0684.2007.00229.x.
Quantitative enzyme-immunoassays of urinary and fecal immunoglobulin A (IgA), cortisol and 11-17-dioxoandrostanes (11,17-DOA), and serum cortisol in eight metabolic-cage-housed female cynomolgus monkeys were performed. The monkeys were divided into two groups, B and NB. Group B animals were blood sampled every 6 hours, whereas Group NB animals were not handled/blood sampled.
No differences were recorded between the amounts of feces and urine excreted by the two groups. Group B animals excreted more urinary cortisol than did Group NB animals indicating that restraint-blood sampling resulted in a stress response. Excreted amounts of IgA and 11,17-DOA (urine and feces) did not differ between the groups.
Urinary cortisol was a reliable marker of the stress associated with repeated blood sampling. Declining amounts of excreted urinary cortisol indicated that cynomolgus monkeys acclimated quickly to repeated blood sampling in metabolism cages. Within and between animal variation in amounts of feces voided demonstrated the importance of expressing fecal markers as 'amounts excreted per time unit per kg body weight' rather than just measuring the concentrations in fecal samples.
对8只饲养在代谢笼中的雌性食蟹猴进行了尿液和粪便免疫球蛋白A（IgA）、皮质醇和11 - 17 - 二氧雄烷（11,17 - DOA）以及血清皮质醇的定量酶免疫测定。这些猴子被分为两组，B组和NB组。B组动物每6小时采血一次，而NB组动物不进行处理/采血。
两组动物排出的粪便和尿液量没有差异。B组动物排出的尿皮质醇比NB组动物多，这表明约束采血会引发应激反应。两组之间IgA和11,17 - DOA（尿液和粪便）的排出量没有差异。
尿皮质醇是与重复采血相关应激的可靠标志物。尿皮质醇排出量的下降表明食蟹猴能迅速适应代谢笼中的重复采血。动物粪便排出量在个体内和个体间的差异表明，将粪便标志物表示为“每千克体重每单位时间排出量”而非仅仅测量粪便样本中的浓度非常重要。
